Piedra Pedro A, Hause Anne M, Aideyan Letisha
Department of Molecular Virology and Microbiology, Baylor College of Medicine, BCM MS280, One Baylor Plaza, Houston, TX, 77030, USA.
Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.
Methods Mol Biol. 2016;1442:77-91. doi: 10.1007/978-1-4939-3687-8_7.
Assays that measure RSV-specific neutralizing antibody activity are very useful for evaluating vaccine candidates, performing seroprevalence studies, and detecting infection. Neutralizing antibody activity is normally measured by a plaque reduction neutralization assay or by a microneutralization assay with or without complement. These assays measure the functional capacity of serum (or other fluids) to neutralize virus infectivity in cells as compared to ELISA assays that only measure the binding capacity against an antigen. This chapter discusses important elements in standardization of the RSV-specific microneutralization assay for use in the laboratory.
检测呼吸道合胞病毒（RSV）特异性中和抗体活性的试验对于评估候选疫苗、开展血清流行率研究以及检测感染情况非常有用。中和抗体活性通常通过蚀斑减少中和试验或有或无补体参与的微量中和试验来测定。与仅测量对抗抗原结合能力的酶联免疫吸附测定（ELISA）试验相比，这些试验测量血清（或其他液体）在细胞中中和病毒感染性的功能能力。本章讨论了在实验室中用于呼吸道合胞病毒特异性微量中和试验标准化的重要因素。
