Ogundero V W, Aina J O
Department of Botany and Microbiology, University of Ibadan, Nigeria.
J Basic Microbiol. 1989;29(3):171-5. doi: 10.1002/jobm.3620290313.
The effects of temperature on storage and viability of asexual spores of Rhizomucor pusillus (Lindt) Schipper and Rhizomucor miehei (Cooney and Emerson) Schipper were studied. An optimal germination temperature of 35 degrees C was recorded for both species with good results obtained around 40 degrees C. On prolonged storage of the spores at various temperatures (-19 degrees C, 4 degrees C, 30 degrees C, 37 degrees C), the best results were obtained at 4 degrees C and 30 degrees C. Very rapid losses in viability of the spores of both fungi occurred at -19 degrees C reaching a maximum of 68% and 51%, respectively, for R. pusillus and R. miehei after 6 months of storage. Continuous exposure of the spores to 68 degrees C led to rapid losses of viability of up to 100% for those of R. pusillus after 40 min exposure. Sporangiospores of R. miehei showed more gradual losses on exposure to 68 degrees C. The potential health hazards which the presence of these fungi on tobacco products pose to man are discussed.
研究了温度对微小根毛霉(Lindt)Schipper和米根毛霉(Cooney和Emerson)Schipper无性孢子储存及活力的影响。两种菌的最佳萌发温度均为35℃,在40℃左右也能取得较好结果。在不同温度(-19℃、4℃、30℃、37℃)下长期储存孢子,4℃和30℃的效果最佳。两种真菌的孢子在-19℃下活力损失非常迅速,储存6个月后,微小根毛霉和米根毛霉的孢子活力分别最高损失68%和51%。将孢子持续暴露于68℃,微小根毛霉的孢子在暴露40分钟后活力迅速损失高达100%。米根毛霉孢囊孢子在暴露于68℃时活力损失较为缓慢。文中讨论了烟草制品上存在这些真菌对人类造成的潜在健康危害。