斑马鱼幼体中宿主-病原体相互作用的活体成像
Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.
作者信息
Matty Molly A, Oehlers Stefan H, Tobin David M
机构信息
Department of Molecular Genetics and Microbiology, Center for Host-Microbial Interactions, Duke University Medical Center, 219 Jones Building, Box 3020 DUMC, Durham, NC, 27710, USA.
University Program in Genetics and Genomics, Duke University, Durham, NC, 27710, USA.
出版信息
Methods Mol Biol. 2016;1451:207-23. doi: 10.1007/978-1-4939-3771-4_14.
Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.
斑马鱼幼体是研究对感染的先天免疫反应的强大平台。幼体斑马鱼体积小且具有光学透明性，这使得进行多对象、多维度和纵向成像实验成为可能。本章描述了用其天然病原体海分枝杆菌感染斑马鱼幼体、快速短期成像、长期扩展成像以及药物治疗分析的实验方案。这些实验方案可以很容易地进行调整，以对宿主与其他病原体的相互作用进行成像和操作。
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