Tuberculosis Research Program at the Centenary Institute, The University of Sydney, Camperdown, NSW, 2050, Australia.
Department of Clinical Immunology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia.
Sci Rep. 2021 Sep 24;11(1):19049. doi: 10.1038/s41598-021-98566-9.
Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.
高血糖会损害微血管，部分原因是免疫细胞的募集减少和血小板信号的干扰，导致哺乳动物伤口愈合不良和脂质沉积加速。我们通过浸泡或注射的方式向斑马鱼幼虫补充外源性葡萄糖，研究了斑马鱼幼虫在模拟外源性葡萄糖对中性粒细胞和巨噬细胞向尾部伤口募集、伤口诱导止血以及鸡卵黄饲料诱导高脂血症的影响中的应用。两种葡萄糖补充方法都不会影响尾部横断后中性粒细胞和巨噬细胞的募集。葡萄糖注射会减少血小板的保留和纤维蛋白塞的形成，而在尾部横断后，只有葡萄糖浸泡会减少血小板的保留。我们观察到在接受高脂肪鸡卵黄喂养的葡萄糖注射幼虫中，脂质积累加速。与哺乳动物相比，我们的研究确定了高葡萄糖对斑马鱼幼虫炎症、止血和高脂血症的保守和差异影响。
