Davison P I, Créach V, Liang W-J, Andreou D, Britton J R, Copp G H
Fisheries and Ecosystems Division, Centre for Environment, Fisheries & Aquaculture Science, Lowestoft, Suffolk, U.K.
Department of Life and Environmental Sciences, Faculty of Science and Technology, Bournemouth University, Bournemouth, Dorset, U.K.
J Fish Biol. 2016 Sep;89(3):1782-93. doi: 10.1111/jfb.13086. Epub 2016 Jul 27.
This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.
本文介绍了一种简单可靠的环境（e）DNA方法开发与验证的第一阶段，该方法使用常规PCR检测四种非本地淡水鱼：湖鲱Lepomis gibbosus、睛斑麦穗鱼Leucaspius delineatus、黑头软口鲦Pimephales promelas和麦穗鱼Pseudorasbora parva。该方法的有效性在室内水箱（44升）试验中得到了证明，在该试验中，所有四种鱼类在24小时内均被检测到。验证通过两项野外试验进行，其中在将湖鲱引入室外实验池塘6-12小时后检测到了该物种，并且在已知存在麦穗鱼的废弃养鱼池塘中成功检测到了麦穗鱼。因此，过滤少量（30毫升）池塘水足以捕获鱼类eDNA，该方法强调了采集具有足够空间覆盖范围的多个水样对于检测随机或斑块分布物种的重要性。
