Rodpai Thanatrinan, Suwannapoom Chatmongkon, Osathanunkul Maslin
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand.
Animals (Basel). 2023 Feb 3;13(3):538. doi: 10.3390/ani13030538.
Siamese bat catfish ( Smith, 1993) has been listed as an endangered species, and its abundance has been severely declining due to habitat degradation and overfishing. To establish an appropriate management strategy, it is crucial to gain information about the distribution of this endangered species. As live under rocks in streams, detecting their presence is difficult. Recently, environmental DNA (eDNA)-based detection has been demonstrated to be a valid tool for monitoring rare species, such as . Therefore, this study developed an eDNA assay targeting a 160 bp fragment of the region to detect the presence of this species in its natural habitat. An amount of 300 mL of water samples (0.7 μm filtered) were collected from 15 sites in the Mae Klang sub-basin, where this fish species was visually detected at two locations. eDNA was detected at 12 of the 15 sites sampled with varying concentrations (0.71-20.27 copies/mL), including at the sites where this species was visually detected previously. The developed eDNA assay was shown to be effective for detecting the presence of this endangered species in the Klang Phat and Klang Rivers within the Doi Inthanon National Park.
暹罗蝙蝠鲶鱼（史密斯，1993年）已被列为濒危物种，由于栖息地退化和过度捕捞，其数量一直在急剧下降。为了制定适当的管理策略，获取有关这种濒危物种分布的信息至关重要。由于它们生活在溪流中的岩石下，很难检测到它们的存在。最近，基于环境DNA（eDNA）的检测已被证明是监测珍稀物种（如……）的有效工具。因此，本研究开发了一种针对……区域160bp片段的eDNA检测方法，以检测该物种在其自然栖息地中的存在。从湄公河次流域的15个地点采集了300mL水样（经0.7μm过滤），在其中两个地点肉眼检测到了这种鱼类。在采样的15个地点中的12个地点检测到了eDNA，其浓度各不相同（0.71 - 20.27拷贝/毫升），包括之前肉眼检测到该物种的地点。所开发的eDNA检测方法被证明对于检测因他农山国家公园内的克拉武河和克拉河中的这种濒危物种的存在是有效的。
