Lan Weiqiu, Wang Wei, Yu Zhimin, Qin Yanxia, Luan Jing, Li Xianzhen
School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian, 116034, People's Republic of China.
Biotechnol Lett. 2016 Nov;38(11):1935-1940. doi: 10.1007/s10529-016-2181-5. Epub 2016 Jul 27.
To study enhanced barley germination by chitooligosaccharide as an elicitor for improving the quality of malt.
Barley germination for both radical and leaf sprouts was enhanced when chitooligosaccharide was added to the steeping water in the first steeping cycle. The activities of hydrolases (α-/β-amylase, proteinase and β-glucanase) and antioxidases (superoxide dismutase and catalase) in the resultant malt were increased in a dose-dependent manner when chitooligosaccharide was supplemented in the steeping water. Maximal promotion was at 1 mg chitooligosaccharide/l for α-/β-amylase and proteinase, and at 10 mg/l for β-glucanase, superoxide dismutase and catalase. Malt quality, including free α-amino nitrogen content, Kolbach index, malt extract content, diastatic power, wort viscosity and the ratio of glucose, maltose and maltotriose, was significantly improved by chitooligosaccharide in seed priming at 1 mg/l.
Application of chitooligosaccharide in the steeping water promotes barley germination and improves the quality of malt.
研究以壳寡糖作为诱导子增强大麦发芽，从而提高麦芽品质。
在首次浸泡循环中，向浸泡水中添加壳寡糖时，大麦的胚根和叶芽发芽均得到增强。当浸泡水中添加壳寡糖时，所得麦芽中水解酶（α-/β-淀粉酶、蛋白酶和β-葡聚糖酶）和抗氧化酶（超氧化物歧化酶和过氧化氢酶）的活性呈剂量依赖性增加。α-/β-淀粉酶和蛋白酶在壳寡糖浓度为1毫克/升时促进作用最大，β-葡聚糖酶、超氧化物歧化酶和过氧化氢酶在10毫克/升时促进作用最大。壳寡糖在种子引发中以1毫克/升的浓度显著改善了麦芽品质，包括游离α-氨基氮含量、库尔巴哈值、麦芽浸出物含量、糖化力、麦汁粘度以及葡萄糖、麦芽糖和麦芽三糖的比例。
在浸泡水中应用壳寡糖可促进大麦发芽并提高麦芽品质。
