Velasco Cristina, Librán-Pérez Marta, Otero-Rodiño Cristina, López-Patiño Marcos A, Míguez Jesús M, Soengas José L
Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, Vigo, Spain.
Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, Vigo, Spain
Am J Physiol Regul Integr Comp Physiol. 2016 Oct 1;311(4):R658-R668. doi: 10.1152/ajpregu.00201.2016. Epub 2016 Jul 27.
We hypothesize that ceramides are involved in the regulation of food intake in fish. Therefore, we assessed in rainbow trout (Oncorhynchus mykiss) the effects of intracerebroventricular treatment with C6:0 ceramide on food intake. In a second experiment, we assessed the effects in brain areas of ceramide treatment on neuropeptide expression, fatty acid-sensing systems, and cellular signaling pathways. Ceramide treatment induced a decrease in food intake, a response opposed to the orexigenic effect described in mammals, which can be related to enhanced mRNA abundance of cocaine and amphetamine-related transcript and proopiomelanocortin and decreased mRNA abundance of Agouti-related protein and neuropeptide Y. Fatty acid-sensing systems appear to be inactivated by ceramide treatment. The mRNA abundance of integrative sensors AMPK and sirtuin 1, and the phosphorylation status of cellular signaling pathways dependent on protein kinase B, AMPK, mammalian target of rapamycin (mTOR), and forkhead box protein O1 (FoxO1) are generally activated by ceramide treatment. However, there are differences between hypothalamus and hindbrain in the phosphorylation status of AMPK (decreased in hypothalamus and increased in hindbrain), mTOR (decreased in hypothalamus and increased in hindbrain), and FoxO1 (increased in hypothalamus and decreased in hindbrain) to ceramide treatment. The results suggest that ceramides are involved in the regulation of food intake in rainbow trout through mechanisms comparable to those characterized previously in mammals in some cases.
我们假设神经酰胺参与鱼类食物摄入量的调节。因此，我们评估了在虹鳟（Oncorhynchus mykiss）中脑室内注射C6:0神经酰胺对食物摄入量的影响。在第二个实验中，我们评估了神经酰胺处理对脑区神经肽表达、脂肪酸感知系统和细胞信号通路的影响。神经酰胺处理导致食物摄入量减少，这一反应与哺乳动物中描述的促食欲作用相反，这可能与可卡因和苯丙胺相关转录物及阿黑皮素原的mRNA丰度增加以及刺鼠相关蛋白和神经肽Y的mRNA丰度降低有关。脂肪酸感知系统似乎因神经酰胺处理而失活。整合传感器AMPK和sirtuin 1的mRNA丰度，以及依赖于蛋白激酶B、AMPK、雷帕霉素靶蛋白（mTOR）和叉头框蛋白O1（FoxO1）的细胞信号通路的磷酸化状态通常因神经酰胺处理而被激活。然而，下丘脑和后脑在AMPK（下丘脑降低，后脑增加）、mTOR（下丘脑降低，后脑增加）和FoxO1（下丘脑增加，后脑降低）对神经酰胺处理的磷酸化状态上存在差异。结果表明，在某些情况下，神经酰胺通过与之前在哺乳动物中所描述的机制类似的机制参与虹鳟食物摄入量的调节。
