Chen Yan, Li Wenyan, Li Wen, Chai Renjie, Li Huawei
Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200031, China.
Central Laboratory, Affiliated Eye and ENT Hospital, Fudan University, Shanghai, 200031, China.
Front Med. 2016 Sep;10(3):330-5. doi: 10.1007/s11684-016-0459-6. Epub 2016 Sep 7.
The enhancer of zeste 2 polycomb repressive complex 2 subunit (Ezh2) is a histone-lysine Nmethyltransferase enzyme that participates in DNA methylation. Ezh2 has also been reported to play crucial roles in stem cell proliferation and differentiation. However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated. Here, we examined the spatiotemporal expression of Ezh2 in the cochlea during embryonic and postnatal development. Ezh2 expression began to be observed in the whole otocyst nuclei at embryonic day 9.5 (E9.5). At E12.5, Ezh2 was expressed in the nuclei of the cochlear prosensory epithelium. At E13.5 and E15.5, Ezh2 was expressed from the apical to the basal turns in the nuclei of the differentiating cochlear epithelium. At postnatal day (P) 0 and 7, the Ezh2 expression was located in the nuclei of the cochlear epithelium in all three turns and could be clearly seen in outer and inner hair cells, supporting cells, the stria vascularis, and spiral ganglion cells. Ezh2 continued to be expressed in the cochlear epithelium of adult mice. Our results provide the basic Ezh2 expression pattern and might be useful for further investigating the detailed role of Ezh2 during cochlear development.
zeste 2多梳抑制复合物2亚基增强子（Ezh2）是一种参与DNA甲基化的组蛋白赖氨酸N甲基转移酶。据报道，Ezh2在干细胞增殖和分化中也起着关键作用。然而，尚未研究Ezh2在小鼠耳蜗发育过程中的详细表达谱。在此，我们研究了Ezh2在胚胎期和出生后发育过程中在耳蜗中的时空表达。在胚胎第9.5天（E9.5），在整个耳囊中开始观察到Ezh2的表达。在E12.5时，Ezh2在耳蜗前感觉上皮细胞核中表达。在E13.5和E15.5时，Ezh2在分化的耳蜗上皮细胞核中从顶端到基底转表达。在出生后第0天（P0）和第7天，Ezh2表达位于所有三个转的耳蜗上皮细胞核中，并且在外毛细胞和内毛细胞、支持细胞、血管纹和螺旋神经节细胞中可以清楚地看到。Ezh2在成年小鼠的耳蜗上皮中持续表达。我们的结果提供了Ezh2的基本表达模式，可能有助于进一步研究Ezh2在耳蜗发育过程中的详细作用。
