环 1B 在新生小鼠耳蜗中的时空表达。

The Spatiotemporal Expression of Ring1B in the Postnatal Mouse Cochlea.

机构信息

Department of Otolaryngology, Shanghai University of Medicine and Health Sciences, Shanghai Jiading Central Hospital, Shanghai, China.

Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Nanchang University, Nanchang, China.

出版信息

J Int Adv Otol. 2022 Jul;18(4):297-301. doi: 10.5152/iao.2022.21473.

DOI:10.5152/iao.2022.21473

PMID:35894525

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9404321/

Abstract

BACKGROUND

Epigenetic regulation plays significant role in the development of the inner ear. It is known that Ring1B can monoubiquitinate H2AK119 to compact nucleosomes and block gene transcription. Ring1B plays crucial role in embryonic development, heterochromatin function, stem cell maintenance and so on. However, it is still unknown whether Ring1B plays a role in the development of inner ear.

METHODS

Here, we used postnatal C56BL/6J mice to examine the expression of Ring1B in the cochlea using real-time quantitative polymerase chain reaction, Western blot, and immunofluorescence staining.

RESULTS

Ring1B mRNA expression was observed at postnatal day 0 and postnatal day 14 cochlea. Ring1B protein was expressed in the cochlea on postnatal days 0, 14, and 30. The Ring1B was observed to be expressed in spiral ganglion cells at postnatal days 0, 14, and 30. Additionally, Ring1B was expressed in hair cells at postnatal days 0, 14, and 30.

CONCLUSION

Our results provide the basic expression pattern of Ring1B and might be helpful for future investigations of the detailed role of Ring1B in the cochlea.

摘要

背景

表观遗传调控在内耳发育中起着重要作用。已知 Ring1B 可以单泛素化 H2AK119，使核小体紧缩并阻断基因转录。Ring1B 在胚胎发育、异染色质功能、干细胞维持等方面发挥着关键作用。然而，目前尚不清楚 Ring1B 是否在内耳发育中发挥作用。

方法

在这里，我们使用出生后 C56BL/6J 小鼠，通过实时定量聚合酶链反应、Western blot 和免疫荧光染色，检测 Ring1B 在耳蜗中的表达。

结果

在出生后第 0 天和第 14 天的耳蜗中观察到 Ring1B mRNA 的表达。在出生后第 0、14 和 30 天的耳蜗中表达 Ring1B 蛋白。在出生后第 0、14 和 30 天，Ring1B 被观察到在螺旋神经节细胞中表达。此外，在出生后第 0、14 和 30 天，Ring1B 在毛细胞中表达。