National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Wens Foodstuff Group Co., Ltd., Yunfu, China.
Biomed Res Int. 2020 Oct 5;2020:5315930. doi: 10.1155/2020/5315930. eCollection 2020.
The proper methylation status of histones is essential for appropriate cell lineage and organogenesis. EZH2, a methyltransferase catalyzing H3K27me3, has been abundantly studied in human and mouse embryonic development. The pig is an increasing important animal model for molecular study and pharmaceutical research. However, the transcript variant and temporal expression pattern of in the middle and late porcine fetus are still unknown. Here, we identified the coding sequence of the gene and characterized its expression pattern in fetal tissues of Duroc pigs at 65- and 90-day postcoitus (dpc). Our results showed that the coding sequence of was 2241 bp, encoding 746 amino acids. There were 9 amino acid insertions and an amino acid substitution in this transcript compared with the validated reference sequence in NCBI. was ubiquitously expressed in the fetal tissues of two time points with different expression levels. These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development.
组蛋白的适当甲基化状态对于适当的细胞谱系和器官发生是必不可少的。EZH2 是一种催化 H3K27me3 的甲基转移酶，在人和小鼠胚胎发育中已有大量研究。猪是分子研究和药物研究中日益重要的动物模型。然而，中晚期猪胎中 的转录变体和时间表达模式仍不清楚。在这里，我们鉴定了 基因的编码序列，并在杜洛克猪交配后 65 天和 90 天（dpc）的胎儿组织中表征了其表达模式。我们的结果表明， 的编码序列为 2241bp，编码 746 个氨基酸。与 NCBI 中验证的参考序列相比，该转录本中有 9 个氨基酸插入和 1 个氨基酸取代。 在两个时间点的胎儿组织中均广泛表达，但表达水平不同。这些结果验证了猪中的不同转录本，并描述了其在不同妊娠阶段胎儿组织中的表达谱，表明 EZH2 在猪胚胎发育过程中发挥了重要作用。
