Gancedo Carlos, Flores Carmen-Lisset, Gancedo Juana M
Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain
Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain.
Microbiol Mol Biol Rev. 2016 Jul 27;80(3):765-77. doi: 10.1128/MMBR.00012-16. Print 2016 Sep.
Moonlighting proteins are multifunctional proteins that participate in unrelated biological processes and that are not the result of gene fusion. A certain number of these proteins have been characterized in yeasts, and the easy genetic manipulation of these microorganisms has been useful for a thorough analysis of some cases of moonlighting. As the awareness of the moonlighting phenomenon has increased, a growing number of these proteins are being uncovered. In this review, we present a crop of newly identified moonlighting proteins from yeasts and discuss the experimental evidence that qualifies them to be classified as such. The variety of moonlighting functions encompassed by the proteins considered extends from control of transcription to DNA repair or binding to plasminogen. We also discuss several questions pertaining to the moonlighting condition in general. The cases presented show that yeasts are important organisms to be used as tools to understand different aspects of moonlighting proteins.
兼性蛋白质是参与不相关生物学过程且并非基因融合产物的多功能蛋白质。其中一些蛋白质已在酵母中得到表征，并且这些微生物易于进行基因操作，这对于深入分析某些兼性情况很有帮助。随着对兼性现象认识的增加，越来越多的此类蛋白质被发现。在本综述中，我们展示了一批新鉴定出的酵母兼性蛋白质，并讨论了使其有资格被归类为此类蛋白质的实验证据。所考虑的蛋白质涵盖的兼性功能种类从转录控制到DNA修复或与纤溶酶原结合不等。我们还讨论了与兼性状态总体相关的几个问题。所呈现的案例表明，酵母是用作理解兼性蛋白质不同方面的工具的重要生物体。
