Department of Metabolism and Cell Signaling, Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-UAM, Madrid, Spain.
IUBMB Life. 2011 Jul;63(7):457-62. doi: 10.1002/iub.454. Epub 2011 Apr 13.
This review considers the use of yeasts to study protein moonlighting functions. The cases discussed highlight the possibilities offered by the well-developed yeast genetics for the study of moonlighting mechanisms. The possibility to generate sets of mutants encoding different protein variants has allowed in some cases to map the regions that participate in the moonlighting function. We discuss cases of enzymes that moonlight in such different activities as control of transcription, assembly of multimeric proteins, stabilization of mitochondrial DNA or biosynthesis of CoA. The moonlighting role of an enzyme and its metabolic function seems to have evolved independently as indicated by the finding that a protein may moonlight in a yeast species but not in others. Yeasts may open ways to study possible evolutionary relationships among moonlighting proteins.
这篇综述探讨了利用酵母来研究蛋白质的双重功能。所讨论的案例突出了酵母遗传学在研究双重功能机制方面提供的可能性。通过生成编码不同蛋白质变体的突变体集合，在某些情况下可以映射参与双重功能的区域。我们讨论了一些酶的案例，这些酶在转录调控、多聚体蛋白组装、线粒体 DNA 稳定或 CoA 生物合成等不同活性中发挥双重功能。酶的双重功能与其代谢功能似乎是独立进化的，因为发现一种蛋白质可能在一种酵母物种中发挥双重功能，但在其他物种中则不然。酵母可能为研究双重功能蛋白之间可能的进化关系开辟道路。
