Cortés-Hinojosa Galaxia, Doescher Bethany, Kinsel Michael, Lednicky John, Loeb Julia, Waltzek Thomas, Wellehan James F X
J Zoo Wildl Med. 2016 Jun;47(2):427-37. doi: 10.1638/2014-0252.1.
The Hawaiian monk seal (Neomonachus schauinslandi) is an endangered species. Here, we present a clinical case of a 26-yr-old male Hawaiian monk seal (HMS) kept in an aquarium with a history of intermittent anorexia and evidence of renal disease. Histologic examination revealed eosinophilic intranuclear inclusions in the liver. Conventional nested PCR protocols were used to test for viruses, and it tested positive for adenovirus and polyomavirus, and negative for herpesvirus. The adenovirus partial polymerase gene is 100% homologous to that of California sea lion adenovirus 1 (CSLAdV-1). CSLAdV-1 causes viral hepatitis in CSL, and has recently been reported in different species of otariids in an aquarium in Japan ( Otaria flavescens and Arctocephalus pusillus ) and a sequence from Spain has been submitted in NCBI as Otaria flavescens adenovirus-1. The polyomavirus in this animal is a novel virus, and is the first polyomavirus discovered in Hawaiian monk seals. This new virus is designated Hawaiian monk seal polyomavirus (HMSPyV-1), and is 83% homologous to California sea lion Polyomavirus-1 (CSLPyV-1). This is the first report of viral coinfection in a HMS and clinical significance in this case remains unclear but may be associated with advanced age.
夏威夷僧海豹（Neomonachus schauinslandi）是一种濒危物种。在此，我们报告一例26岁雄性夏威夷僧海豹（HMS）的临床病例，该海豹饲养在水族馆，有间歇性厌食病史且有肾病迹象。组织学检查显示肝脏中有嗜酸性核内包涵体。采用传统的巢式PCR方案检测病毒，结果显示腺病毒和多瘤病毒呈阳性，疱疹病毒呈阴性。腺病毒部分聚合酶基因与加州海狮腺病毒1（CSLAdV - 1）的100%同源。CSLAdV - 1可导致加州海狮发生病毒性肝炎，最近在日本一家水族馆的不同种海狗科动物（南美海狗和南美毛皮海狮）中也有报道，并且来自西班牙的一个序列已作为南美海狗腺病毒 - 1提交至NCBI。该动物体内的多瘤病毒是一种新型病毒，是在夏威夷僧海豹中发现的首例多瘤病毒。这种新病毒被命名为夏威夷僧海豹多瘤病毒（HMSPyV - 1），与加州海狮多瘤病毒 - 1（CSLPyV - 1）的同源性为83%。这是关于夏威夷僧海豹病毒合并感染的首例报告，该病例的临床意义尚不清楚，但可能与年龄较大有关。
