Dikshit H K, Singh Akanksha, Singh D, Aski M, Jain Neelu, Hegde V S, Basandrai A K, Basandrai D, Sharma T R
Indian J Exp Biol. 2016 Jun;54(6):394-9.
Lentil, as an economical source of protein, minerals and vitamins, plays important role in nutritional security of the common man. Grown mainly in West Asia, North Africa (WANA) region and South Asia, it suffers from several biotic stresses such as wilt, rust, blight and broomrape. Lentil rust caused by autoecious fungus Uromyces viciae fabae (Pers.) Schroet is a serious lentil disease in Algeria, Bangladesh, Ethiopia, India, Italy, Morocco, Pakistan and Nepal. The disease symptoms are observed during flowering and early podding stages. Rust causes severe yield losses in lentil. It can only be effectively controlled by identifying the resistant source, understanding its inheritance and breeding for host resistance. The obligate parasitic nature of pathogen makes it difficult to maintain the pathogen in culture and to apply it to screen segregating progenies under controlled growth conditions. Hence, the use of molecular markers will compliment in identification of resistant types in different breeding programs. Here, we studied the inheritance of resistance to rust in lentil using F₁, F₂ and F₂:₃ from cross PL 8 (susceptible) x L 4149 (resistant) varieties. The phenotyping of lentil population was carried out at Sirmour, India. The result of genetic analysis revealed that a single dominant gene controls rust resistance in lentil genotype L 4149. The F2 population from this cross was used to tag and map the rust resistance gene using SSR and SRAP markers. Markers such as 270 SRAP and 162 SSR were studied for polymorphism and 101 SRAP and 33 SSRs were found to be polymorphic between the parents. Two SRAP and two SSR markers differentiated the resistant and susceptible bulks. SSR marker Gllc 527 was estimated to be linked to rust resistant locus at a distance of 5.9 cM. The Gllc 527 marker can be used for marker assisted selection for rust resistance; however, additional markers closer to rust resistant locus are required. The markers linked to the rust resistance gene can serve as starting points for map-based cloning of the rust resistance gene.
小扁豆作为一种经济的蛋白质、矿物质和维生素来源，在普通人的营养安全中发挥着重要作用。它主要生长在西亚、北非（WANA）地区和南亚，遭受多种生物胁迫，如枯萎病、锈病、疫病和列当。由自生性真菌蚕豆单胞锈菌（Uromyces viciae fabae (Pers.) Schroet）引起的小扁豆锈病，在阿尔及利亚、孟加拉国、埃塞俄比亚、印度、意大利、摩洛哥、巴基斯坦和尼泊尔是一种严重的小扁豆病害。病害症状在开花期和结荚初期出现。锈病会导致小扁豆严重减产。只有通过鉴定抗性来源、了解其遗传特性并培育寄主抗性才能有效控制该病。病原菌的专性寄生性质使得在培养中维持病原菌并在受控生长条件下应用于筛选分离后代变得困难。因此，分子标记的使用将有助于在不同育种计划中鉴定抗性类型。在此，我们利用杂交组合PL 8（感病）×L 4149（抗病）品种的F₁、F₂和F₂:₃研究了小扁豆对锈病抗性的遗传。小扁豆群体的表型分析在印度锡尔穆尔进行。遗传分析结果表明，单个显性基因控制小扁豆基因型L 4149中的锈病抗性。该杂交组合的F2群体用于使用SSR和SRAP标记对锈病抗性基因进行标记和定位。研究了270个SRAP和162个SSR标记的多态性，发现101个SRAP和33个SSR在亲本之间具有多态性。两个SRAP和两个SSR标记区分了抗病和感病混合群体。SSR标记Gllc 527估计与锈病抗性位点连锁，距离为5.9厘摩。Gllc 527标记可用于锈病抗性的标记辅助选择；然而，需要更接近锈病抗性位点的其他标记。与锈病抗性基因连锁 的标记可作为基于图谱克隆锈病抗性基因的起点。
