Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
Nanoscale. 2016 Sep 28;8(36):16221-8. doi: 10.1039/c6nr04469g. Epub 2016 Jul 29.
A metal-organic molecular net composed of tannic acid (TA) and iron(iii) was constructed around the brome mosaic virus (BMV) particle to determine whether the added net could act as a transport barrier for water, and if the net could stabilize the virus in physically or chemically challenging environments. This new virus engineering strategy is expected to provide benefits both in the study and technological applications of viruses. For instance, a virus wrapped in a thin molecular layer could be extracted from solution either in air or vacuum, and its structure, composition and even internal dynamics could be interrogated by methods not compatible with a liquid environment. Atomic force microscopy (AFM) studies of Fe(iii)-TA coated BMV in liquid and in air supported a marked resistance to dehydration when compared to wtBMV. Native charge detection mass spectrometry (CDMS), was employed to estimate the number of molecules in the molecular net which wrapped the virus. The CDMS data suggested that less than one molecular monolayer wrapped the virus. Additionally, it was found, that this very thin molecular coat was sufficient to render the coated viruses resistant to storage conditions that typically lead to virus disassembly over time. A temporary coat imparting increased resistance to disassembly could be useful in adding time delay control or alleviate required storage conditions of engineered viruses for therapeutic purposes.
构建了由单宁酸（TA）和三价铁组成的金属有机分子网络，围绕着野油菜 mosaic 病毒（BMV）颗粒，以确定添加的网络是否可以作为水的传输屏障，以及网络是否可以稳定物理或化学挑战性环境中的病毒。这种新的病毒工程策略有望在病毒的研究和技术应用中带来好处。例如，包裹在薄分子层中的病毒可以从溶液中提取，无论是在空气还是真空中，其结构、组成甚至内部动力学都可以通过与液体环境不兼容的方法进行检测。在液体和空气中对 Fe(iii)-TA 涂层 BMV 的原子力显微镜（AFM）研究表明，与 wtBMV 相比，其脱水阻力明显增强。采用天然电荷检测质谱（CDMS）来估计包裹病毒的分子网络中的分子数量。CDMS 数据表明，不到一个分子单层包裹了病毒。此外，还发现，这种非常薄的分子涂层足以使涂覆的病毒能够抵抗通常会导致病毒随时间解体的储存条件。赋予增加的抗解体能力的临时涂层可能有助于添加延迟控制或减轻工程病毒的所需储存条件以用于治疗目的。
