• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

参与神经退行性疾病的淀粉样纤维的质量和电荷分布：绘制异质性和多态性图谱。

Mass and charge distributions of amyloid fibers involved in neurodegenerative diseases: mapping heterogeneity and polymorphism.

作者信息

Pansieri Jonathan, Halim Mohammad A, Vendrely Charlotte, Dumoulin Mireille, Legrand François, Sallanon Marcelle Moulin, Chierici Sabine, Denti Simona, Dagany Xavier, Dugourd Philippe, Marquette Christel, Antoine Rodolphe, Forge Vincent

机构信息

Univ. Grenoble Alpes , CNRS , CEA , BIG/CBM/AFFOND , F-38000 Grenoble , France . Email:

Institut Lumière Matière , UMR 5306 , Université Claude Bernard Lyon 1 , CNRS , F-69622 Lyon , France . Email:

出版信息

Chem Sci. 2018 Feb 5;9(10):2791-2796. doi: 10.1039/c7sc04542e. eCollection 2018 Mar 14.

DOI:10.1039/c7sc04542e
PMID:29732065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5914292/
Abstract

Heterogeneity and polymorphism are generic features of amyloid fibers with some important effects on the related disease development. We report here the characterization, by charge detection mass spectrometry, of amyloid fibers made of three polypeptides involved in neurodegenerative diseases: Aβ peptide, tau and α-synuclein. Beside the mass of individual fibers, this technique enables to characterize the heterogeneity and the polymorphism of the population. In the case of Aβ peptide and tau protein, several coexisting species could be distinguished and characterized. In the case of α-synuclein, we show how the polymorphism affects the mass and charge distributions.

摘要

异质性和多态性是淀粉样纤维的普遍特征，对相关疾病的发展有一些重要影响。我们在此报告通过电荷检测质谱对由三种与神经退行性疾病相关的多肽（β-淀粉样肽、tau蛋白和α-突触核蛋白）构成的淀粉样纤维的表征。除了单个纤维的质量外，该技术还能够表征群体的异质性和多态性。对于β-淀粉样肽和tau蛋白，可以区分和表征几种共存的物种。对于α-突触核蛋白，我们展示了多态性如何影响质量和电荷分布。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/ea2529f7292a/c7sc04542e-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/815295c27514/c7sc04542e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/de280081b22d/c7sc04542e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/a80615dce7dd/c7sc04542e-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/ea2529f7292a/c7sc04542e-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/815295c27514/c7sc04542e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/de280081b22d/c7sc04542e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/a80615dce7dd/c7sc04542e-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a4a0/5914292/ea2529f7292a/c7sc04542e-f4.jpg

相似文献

1
Mass and charge distributions of amyloid fibers involved in neurodegenerative diseases: mapping heterogeneity and polymorphism.参与神经退行性疾病的淀粉样纤维的质量和电荷分布：绘制异质性和多态性图谱。
Chem Sci. 2018 Feb 5;9(10):2791-2796. doi: 10.1039/c7sc04542e. eCollection 2018 Mar 14.
2
Association of cerebrospinal fluid β-amyloid 1-42, T-tau, P-tau181, and α-synuclein levels with clinical features of drug-naive patients with early Parkinson disease.脑脊液β-淀粉样蛋白 1-42、T 蛋白、P 蛋白 181 和α-突触核蛋白水平与未经药物治疗的早期帕金森病患者临床特征的关系。
JAMA Neurol. 2013 Oct;70(10):1277-87. doi: 10.1001/jamaneurol.2013.3861.
3
The Role of Amyloid-β, Tau, and α-Synuclein Proteins as Putative Blood Biomarkers in Patients with Cerebral Amyloid Angiopathy.淀粉样β、tau 和 α-突触核蛋白作为脑淀粉样血管病患者的潜在血液生物标志物的作用。
J Alzheimers Dis. 2022;89(3):1039-1049. doi: 10.3233/JAD-220216.
4
Longitudinal striatal dopamine transporter binding and cerebrospinal fluid alpha-synuclein, amyloid beta, total tau, and phosphorylated tau in Parkinson's disease.帕金森病患者纹状体多巴胺转运体纵向结合与脑脊液α-突触核蛋白、淀粉样β、总tau 和磷酸化 tau。
Neurol Sci. 2023 Feb;44(2):573-585. doi: 10.1007/s10072-022-06440-x. Epub 2022 Oct 13.
5
REM sleep behavior disorder and cerebrospinal fluid alpha-synuclein, amyloid beta, total tau and phosphorylated tau in Parkinson's disease: a cross-sectional and longitudinal study.帕金森病中 REM 睡眠行为障碍与脑脊液α-突触核蛋白、淀粉样β、总tau 和磷酸化 tau 的关系：一项横断面和纵向研究。
J Neurol. 2022 Sep;269(9):4836-4845. doi: 10.1007/s00415-022-11120-z. Epub 2022 Apr 15.
6
Broad neutralization of calcium-permeable amyloid pore channels with a chimeric Alzheimer/Parkinson peptide targeting brain gangliosides.用靶向脑苷脂的嵌合阿尔茨海默病/帕金森病肽对钙通透性淀粉样蛋白孔通道进行广泛中和。
Biochim Biophys Acta. 2016 Feb;1862(2):213-22. doi: 10.1016/j.bbadis.2015.11.012. Epub 2015 Dec 2.
7
α-Synuclein Heterocomplexes with β-Amyloid Are Increased in Red Blood Cells of Parkinson's Disease Patients and Correlate with Disease Severity.帕金森病患者红细胞中与β-淀粉样蛋白形成的α-突触核蛋白异源复合物增加，并与疾病严重程度相关。
Front Mol Neurosci. 2018 Feb 22;11:53. doi: 10.3389/fnmol.2018.00053. eCollection 2018.
8
Mechanical stress increases brain amyloid β, tau, and α-synuclein concentrations in wild-type mice.机械应力增加野生型小鼠脑内淀粉样 β、tau 和 α-突触核蛋白的浓度。
Alzheimers Dement. 2018 Apr;14(4):444-453. doi: 10.1016/j.jalz.2017.11.003. Epub 2017 Dec 12.
9
Amyloid β, Tau, and α-Synuclein aggregates in the pathogenesis, prognosis, and therapeutics for neurodegenerative diseases.淀粉样β、Tau 和 α-突触核蛋白聚集在神经退行性疾病的发病机制、预后和治疗中的作用。
Prog Neurobiol. 2022 Jul;214:102270. doi: 10.1016/j.pneurobio.2022.102270. Epub 2022 Apr 18.
10
Plasma extracellular vesicles tau and β-amyloid as biomarkers of cognitive dysfunction of Parkinson's disease.血浆细胞外囊泡tau 和 β-淀粉样蛋白作为帕金森病认知功能障碍的生物标志物。
FASEB J. 2021 Oct;35(10):e21895. doi: 10.1096/fj.202100787R.

引用本文的文献

1
Recent Advances in Amyloids Structural Studies and Thin Film Applications.淀粉样蛋白结构研究与薄膜应用的最新进展
Molecules. 2025 Jul 9;30(14):2908. doi: 10.3390/molecules30142908.
2
Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers.在轨道阱分析仪上进行多重电荷检测质谱分析的标准化工作流程。
Nat Protoc. 2025 Jan 2. doi: 10.1038/s41596-024-01091-y.
3
Detecting the Undetectable: Advances in Methods for Identifying Small Tau Aggregates in Neurodegenerative Diseases.检测难以检测到的物质：神经退行性疾病中小tau蛋白聚集体鉴定方法的进展

本文引用的文献

1
Single Particle Analyzer of Mass: A Charge Detection Mass Spectrometer with a Multi-Detector Electrostatic Ion Trap.单粒子质量分析仪：一种带有多探测器静电离子阱的电荷检测质谱仪。
Int J Mass Spectrom. 2017 Mar;414:45-55. doi: 10.1016/j.ijms.2017.01.007. Epub 2017 Jan 15.
2
Structural variation in amyloid-β fibrils from Alzheimer's disease clinical subtypes.阿尔茨海默病临床亚型中淀粉样β纤维的结构变异
Nature. 2017 Jan 12;541(7636):217-221. doi: 10.1038/nature20814. Epub 2017 Jan 4.
3
Native Mass Spectrometry: What is in the Name?Native Mass Spectrometry：名称中蕴含的意义
Chembiochem. 2025 Apr 1;26(7):e202400877. doi: 10.1002/cbic.202400877. Epub 2025 Jan 8.
4
Electrostatic Linear Ion Trap Optimization Strategy for High Resolution Charge Detection Mass Spectrometry.用于高分辨率电荷检测质谱的静电线性离子阱优化策略
J Am Soc Mass Spectrom. 2023 Aug 2;34(8):1731-1740. doi: 10.1021/jasms.3c00177. Epub 2023 Jul 19.
5
Mass spectrometric insights into protein aggregation.质谱法对蛋白质聚集的深入了解。
Essays Biochem. 2023 Mar 29;67(2):243-253. doi: 10.1042/EBC20220103.
6
Influence of Centrifugation and Shaking on the Self-Assembly of Lysozyme Fibrils.离心和振荡对溶菌酶原纤维自组装的影响。
Biomolecules. 2022 Nov 24;12(12):1746. doi: 10.3390/biom12121746.
7
Using mass spectrometry-based methods to understand amyloid formation and inhibition of alpha-synuclein and amyloid beta.利用基于质谱的方法来了解α-突触核蛋白和淀粉样β的淀粉样形成和抑制。
Mass Spectrom Rev. 2024 Jul-Aug;43(4):782-825. doi: 10.1002/mas.21814. Epub 2022 Oct 12.
8
Charge detection mass spectrometry for the analysis of viruses and virus-like particles.电荷检测质谱法用于病毒和类病毒粒子的分析。
Essays Biochem. 2023 Mar 29;67(2):315-323. doi: 10.1042/EBC20220101.
9
Mass Spectrometry Methods for Measuring Protein Stability.质谱法测量蛋白质稳定性。
Chem Rev. 2022 Apr 27;122(8):7690-7719. doi: 10.1021/acs.chemrev.1c00857. Epub 2022 Mar 22.
10
Applications of Charge Detection Mass Spectrometry in Molecular Biology and Biotechnology.电荷检测质谱在分子生物学和生物技术中的应用。
Chem Rev. 2022 Apr 27;122(8):7415-7441. doi: 10.1021/acs.chemrev.1c00377. Epub 2021 Oct 12.
J Am Soc Mass Spectrom. 2017 Jan;28(1):5-13. doi: 10.1007/s13361-016-1545-3. Epub 2016 Dec 1.
4
Measurement of the accurate mass of a 50 MDa infectious virus.50兆道尔顿感染性病毒准确质量的测量。
Rapid Commun Mass Spectrom. 2016 Sep 15;30(17):1957-62. doi: 10.1002/rcm.7673.
5
Catching a virus in a molecular net.在分子网中捕获病毒。
Nanoscale. 2016 Sep 28;8(36):16221-8. doi: 10.1039/c6nr04469g. Epub 2016 Jul 29.
6
Resolving Adeno-Associated Viral Particle Diversity With Charge Detection Mass Spectrometry.解析腺相关病毒颗粒多样性的电荷检测质谱法。
Anal Chem. 2016 Jul 5;88(13):6718-25. doi: 10.1021/acs.analchem.6b00883. Epub 2016 Jun 16.
7
Quantitative thermophoretic study of disease-related protein aggregates.疾病相关蛋白质聚集体的定量热泳研究
Sci Rep. 2016 Mar 17;6:22829. doi: 10.1038/srep22829.
8
Polymorphism of Amyloid Fibrils In Vivo.体内淀粉样纤维的多态性。
Angew Chem Int Ed Engl. 2016 Apr 4;55(15):4822-5. doi: 10.1002/anie.201511524. Epub 2016 Mar 8.
9
MpUL-multi: Software for Calculation of Amyloid Fibril Mass per Unit Length from TB-TEM Images.MpUL-multi：用于从透射电子显微镜（TB-TEM）图像计算每单位长度淀粉样纤维质量的软件。
Sci Rep. 2016 Feb 12;6:21078. doi: 10.1038/srep21078.
10
Mass Determination of Entire Amyloid Fibrils by Using Mass Spectrometry.利用质谱法对整个淀粉样纤维进行质量测定。
Angew Chem Int Ed Engl. 2016 Feb 12;55(7):2340-4. doi: 10.1002/anie.201508995. Epub 2015 Dec 23.