Department of Respiratory Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080, China.
School of Pharmaceutical Science, Southern Medical University, Guangzhou, 510515, China.
Inflammation. 2016 Oct;39(5):1757-63. doi: 10.1007/s10753-016-0410-7.
The aim of this study is to investigate the effects of dexpanthenol in a model of acute lung injury (ALI) induced by lipopolysaccharides (LPS). Lung injury was induced by exposure to atomized LPS. Mice were randomly divided into four groups: control group; Dxp (500 mg/kg) group; LPS group; LPS + Dxp (500 mg/kg) group. The effects of dexpanthenol on LPS-induced neutrophil recruitment, cytokine levels, total protein concentration, myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) contents were examined. Additionally, lung tissue was examined by histology to investigate the changes in pathology in the presence and absence of dexpanthenol. In LPS-challenged mice, dexpanthenol significantly improved lung edema. Dexpanthenol also markedly inhibited the LPS-induced neutrophiles influx, protein leakage, and release of TNF-α and IL-6 in bronchoalveolar lavage fluid (BALF). Furthermore, dexpanthenol attenuated MPO activity and MDA contents and increased SOD and GSH activity in the LPS-challenged lung tissue. These data suggest that dexpanthenol protects mice from LPS-induced acute lung injury by its anti-inflammatory and anti-oxidative activities.
本研究旨在探讨泛醇在脂多糖（LPS）诱导的急性肺损伤（ALI）模型中的作用。通过雾化 LPS 诱导肺损伤。将小鼠随机分为四组：对照组；Dxp（500mg/kg）组；LPS 组；LPS+Dxp（500mg/kg）组。考察了泛醇对 LPS 诱导的中性粒细胞募集、细胞因子水平、总蛋白浓度、髓过氧化物酶（MPO）、丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽（GSH）含量的影响。此外，通过组织病理学检查肺组织，研究有无泛醇时病理变化的变化。在 LPS 攻击的小鼠中，泛醇显著改善了肺水肿。泛醇还明显抑制了 LPS 诱导的中性粒细胞浸润、蛋白渗漏以及支气管肺泡灌洗液（BALF）中 TNF-α和 IL-6 的释放。此外，泛醇减弱了 LPS 攻击肺组织中的 MPO 活性和 MDA 含量，并增加了 SOD 和 GSH 活性。这些数据表明，泛醇通过抗炎和抗氧化活性保护 LPS 诱导的急性肺损伤的小鼠。
