Park Sooyeon, Park Ji-Min, Lee Kang Hyun, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115 Yuseong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Oct;66(10):4172-4178. doi: 10.1099/ijsem.0.001331. Epub 2016 Jul 27.
A Gram-stain-negative, aerobic, non-flagellated, motile by gliding and ovoid or rod-shaped bacterial strain, designated YKTF-7T, was isolated from a tidal flat sediment on the Yellow Sea, South Korea. Strain YKTF-7T grew optimally at pH 7.0-8.0, at 30-37 °C and in the presence of 0.5-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain YKTF-7T fell within the clade comprising the type strains of species of the genus Pontibacter, clustering with the type strains of Pontibacterodishensis and Pontibacterkorlensis with which it exhibited 98.0-98.7 % sequence similarity. Strain YKTF-7T exhibited 16S rRNA gene sequence similarities of 94.7-96.8 % to the type strains of the other species of the genus Pontibacter and of less than 91.7 % to those of other recognized species. Strain YKTF-7T contained MK-7 as the predominant menaquinone and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B) and iso-C15 : 0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain YKTF-7T was 54.0 mol%, and its mean DNA-DNA relatedness values with the type strains of P. odishensis and P. korlensis were 25 and 14 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain YKTF-7T is separated from recognized species of the genus Pontibacter. On the basis of the data presented, strain YKTF-7T is considered to represent a novel species of the genus Pontibacter, for which the name Pontibacterlitorisediminis sp. nov. is proposed. The type strain is YKTF-7T(=KCTC 52252T=NBRC 112298T).
从韩国黄海潮滩沉积物中分离出一株革兰氏染色阴性、需氧、无鞭毛、靠滑动运动的卵形或杆状细菌菌株，命名为YKTF-7T。菌株YKTF-7T在pH 7.0 - 8.0、30 - 37 °C以及0.5 - 2.0 %（w/v）NaCl条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株YKTF-7T属于包含Pontibacter属各物种模式菌株的进化枝，与Pontibacter odishensis和Pontibacter korlensis的模式菌株聚类，序列相似性为98.0 - 98.7 %。菌株YKTF-7T与Pontibacter属其他物种的模式菌株16S rRNA基因序列相似性为94.7 - 96.8 %，与其他已确认物种的模式菌株相似性低于91.7 %。菌株YKTF-7T以MK-7作为主要甲基萘醌，以特征性总和4（异-C17 : 1 I和/或anteiso-C17 : 1 B）和异-C15 : 0作为主要脂肪酸。主要极性脂为磷脂酰乙醇胺和两种未鉴定的脂类。菌株YKTF-7T的DNA G+C含量为54.0 mol%，其与P. odishensis和P. korlensis模式菌株的平均DNA-DNA相关性值分别为25%和14 %。不同的表型特征，以及系统发育和遗传独特性表明，菌株YKTF-7T与Pontibacter属已确认的物种不同。根据所提供的数据，菌株YKTF-7T被认为代表Pontibacter属的一个新物种，为此提出Pontibacter litorisediminis sp. nov.的名称。模式菌株为YKTF-7T(=KCTC 52252T=NBRC 112298T)。
