Park Sooyeon, Choi Jeehyun, Choi Su Jung, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Feb;68(2):630-635. doi: 10.1099/ijsem.0.002555. Epub 2018 Jan 5.
A Gram-stain-negative, facultatively aerobic, motile-by-gliding, non-flagellated and rod-shaped bacterial strain, designated YSM-43, was isolated from a tidal flat in Yeosu on the South Sea in the Republic of Korea. Strain YSM-43 grew optimally at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain YSM-43 fell within the clade comprising type strains of Flavobacterium species, clustering with the type strains of Flavobacterium jejuense and Flavobacterium jumunjinense. It exhibited 16S rRNA gene sequence similarity values of 97.20 and 97.15 % to the type strains of F. jejuense and F. jumunjinense, respectively, and of less than 96.59 % to the type strains of the other Flavobacterium species. Strain YSM-43 contained menaquinone-6 as the predominant menaquinone and iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain YSM-43 was 29.8 mol% and its DNA-DNA relatedness values with type strains of F. jejuense and F. jumunjinense were 13 and 11 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain YSM-43 is separate from other recognized species of the genus Flavobacterium. On the basis of the data presented, strain YSM-43 is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacteriumsediminilitoris sp. nov. is proposed. The type strain is YSM-43 (=KACC 19435=KCTC 62142=NBRC 113020).
从韩国南海丽水的潮滩中分离出一株革兰氏染色阴性、兼性需氧、靠滑行运动、无鞭毛的杆状细菌，命名为YSM-43。菌株YSM-43在30 °C和1.0-2.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株YSM-43属于包含黄杆菌属模式菌株的进化枝，与济州黄杆菌和巨文岛黄杆菌的模式菌株聚类。它与济州黄杆菌和巨文岛黄杆菌模式菌株的16S rRNA基因序列相似性值分别为97.20%和97.15 %，与其他黄杆菌属模式菌株的相似性值低于96.59 %。菌株YSM-43以甲基萘醌-6作为主要甲基萘醌，以异-C15 : 0、异-C17 : 0 3-OH、异-C15 : 1 G和异-C15 : 0 3-OH作为主要脂肪酸。主要极性脂为磷脂酰乙醇胺和一种未鉴定的脂类。菌株YSM-43的DNA G+C含量为29.8 mol%，其与济州黄杆菌和巨文岛黄杆菌模式菌株的DNA-DNA相关性值分别为13%和11 %。这些不同的表型特征，连同系统发育和遗传数据，表明菌株YSM-43与黄杆菌属其他已确认的物种不同。根据所提供的数据，菌株YSM-43被认为代表黄杆菌属的一个新物种，为此提议将其命名为滨海黄杆菌（Flavobacterium sediminilitoris sp. nov.）。模式菌株为YSM-43（=KACC 19435=KCTC 62142=NBRC 113020）。
