School of Medicine and Surgery, University of Milan-Bicocca, Milan, Italy.
Ecole Dentaire Garancière, University of Paris Diderot, France.
J Biol Regul Homeost Agents. 2016 Apr-Jun;30(2 Suppl 1):87-97.
In periodontology, lasers have been suggested for the photodynamic therapy (PDT). Such therapy can be defined as the inactivation of cells, microorganisms or molecules induced by light and not by heat. The aim of our study is to assess the effect of Oxygen high-level laser therapy (OHLLT) in removing all bacterial deposits on root or implant surface by means of mechanical instrumentation and laser irradiation. OHLLT has two effects on targeted bacteria and tissues, decontamination and biostimulation. A total of 33 patients were randomly selected with a diagnosis of chronic periodontitis. The patients enrolled were 16 females and 17 males, six smokers and 4 diabetic patients. For each patient a periodontal charting was performed, assessing probing depth, plaque index and bleeding on probing at baseline and after 6 months. Microbiological analysis were performed with PCR Real Time, using paper tips to withdraw gingival fluid in periodontal pockets before and after treatment, at baseline and after 6 months. All patients were treated with OHLLT at baseline, after 1 week, after 2 weeks and every month for 6 months. After 6 months, all periodontal pockets were treated successfully, without complications and no significant differences in results. All clinical parameters showed an improvement, with a decrease both of plaque index (average decrease of 75%), bleeding on probing (average decrease of 62%) and probing depth (average decrease of 1.8 mm). After the treatment, a remarkable decrease in bacteria amount, both for each species and for total bacteria was observed except for Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis demonstrating that this laser protocol is effective on periodontitis treatment. OHLLT is efficient in treatment of chronic periodontitis as demonstrated by clinical and microbiological parameters, going beyond the traditional periodontal therapy.
在牙周病学中，激光已被提议用于光动力疗法（PDT）。这种疗法可以定义为通过光而不是热诱导的细胞、微生物或分子失活。我们的研究目的是评估高氧激光疗法（OHLLT）在通过机械仪器和激光照射去除根或植入物表面所有细菌沉积物方面的效果。OHLLT 对靶向细菌和组织有两种作用，即去污和生物刺激。总共随机选择了 33 名患有慢性牙周炎的患者。入选的患者有 16 名女性和 17 名男性，6 名吸烟者和 4 名糖尿病患者。每位患者都进行了牙周图表记录，在基线和 6 个月后评估探诊深度、菌斑指数和探诊出血。使用聚合酶链反应实时（PCR Real Time）进行微生物分析，使用纸质尖端在治疗前后从牙周袋中提取牙龈液，在基线和 6 个月后。所有患者在基线时、治疗后 1 周、2 周和治疗后 6 个月每月接受 OHLLT 治疗。6 个月后，所有牙周袋均成功治疗，无并发症，结果无显著差异。所有临床参数均有所改善，菌斑指数（平均降低 75%）、探诊出血（平均降低 62%）和探诊深度（平均降低 1.8 毫米）均降低。治疗后，观察到细菌数量明显减少，每种细菌和总细菌的数量都减少了，除了伴放线放线杆菌和牙龈卟啉单胞菌，这表明这种激光方案对牙周炎的治疗有效。OHLLT 在慢性牙周炎的治疗中有效，如临床和微生物学参数所示，超出了传统的牙周治疗。
