Department of Medicine and Surgery, University of Milan-Bicocca, Milan, Italy.
Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
J Biol Regul Homeost Agents. 2016 Apr-Jun;30(2 Suppl 1):149-54.
The aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in Italian patients affected by chronic periodontitis. The sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the American Academy of Periodontology sampled in the period 2013-2015; 1,323 patients were from Northern Italy, 317 from Central Italy and 122 from Southern Italy. Samples for microbiological analysis were collected from the four sites of the greatest probing depth in each patient and then processed by quantitative polymerase chain reaction. Periodontal pathogens have the following percentage respect to total bacteria load: Aggregatibacter actinomycetemcomitans 0.1%, Campylobacter rectus 2%, Fusobacterium nucleatum 8%, Porphyromonas gingivalis 6%, Treponema denticola 2% and Tannerella forsythia 1.5%. There are significant differences in bacterial load among the different geographical areas both for the total bacterial and for the single species. The results of our study in this Italian population showed that a different geographic distribution exists among periodontal pathogens. We hypothesize that these differences in bacterial load could be related to genetic and environmental factors. Additional studies are necessary to confirm these data and to get more insight on additional factors, which may play a role in periodontal pathogens in different geographic areas.
本研究旨在评估意大利慢性牙周炎患者牙周致病菌的平均细菌负荷。该样本包括 2013 年至 2015 年期间根据美国牙周病学会标准临床诊断为慢性牙周炎的 1762 名患者；其中 1323 名来自意大利北部，317 名来自意大利中部，122 名来自意大利南部。从每位患者最大探诊深度的四个部位采集微生物分析样本，然后通过定量聚合酶链反应进行处理。牙周致病菌占总细菌负荷的以下百分比：伴放线放线杆菌 0.1%，直肠弯曲杆菌 2%，核梭杆菌 8%，牙龈卟啉单胞菌 6%，齿密螺旋体 2%和福赛坦纳氏菌 1.5%。不同地理区域之间的总细菌和单个物种的细菌负荷存在显著差异。我们在意大利人群中的这项研究结果表明，牙周致病菌存在不同的地理分布。我们假设这些细菌负荷的差异可能与遗传和环境因素有关。需要进一步的研究来证实这些数据，并进一步了解可能在不同地理区域的牙周致病菌中起作用的其他因素。
