Kojima H, Ohi H, Seki M, Sakamoto K, Hatano M, Sudou S, Sudou Y
Nihon Jinzo Gakkai Shi. 1989 Jan;31(1):91-7.
Incubation of normal human serum with hemodialysis membranes in vitro resulted in conversion of C3 which was detected by crossed immunoelectrophoresis. In addition, formation of C3d was also observed, as was detected by double-decker rocket immunoelectrophoresis. Furthermore, breakdown products of complement (iC3b, C4d & Bb) in plasma samples were measured by ELISA. The microassay plates were coated with monoclonal antibodies which bind specifically to human iC3b, C4d and Bb, respectively, while the plasma samples were drawn from five polycystic kidney patients during initial hemodialysis. As a result, the iC3b and Bb levels in plasma were seen to increase during hemodialysis but the C4d levels revealed no significant changes. It was also observed that the Bb levels in patients undergoing hemodialysis were significantly higher than those in various renal and collagen diseases. Terminal complement complex (TCC) was not present in detectable amounts in normal human serum when measured by ELISA. However, incubation of normal human serum with hemodialysis membranes in vitro resulted in detection of TCC. TCC was present in the same plasma samples drawned from the five polycystic kidney patients during initial hemodialysis. It is suggestive that activation of complement by hemodialysis membrane is predominantly mediated through the alternative pathway and products such as TCC and anaphylatoxin are formed by this activation.
正常人血清与血液透析膜在体外孵育，通过交叉免疫电泳检测到C3发生了转化。此外，通过双层火箭免疫电泳检测到还形成了C3d。此外，采用酶联免疫吸附测定法（ELISA）检测血浆样本中补体的裂解产物（iC3b、C4d和Bb）。微孔板分别包被与人类iC3b、C4d和Bb特异性结合的单克隆抗体，血浆样本取自5例多囊肾患者首次血液透析期间。结果显示，血液透析期间血浆中iC3b和Bb水平升高，但C4d水平无显著变化。还观察到，接受血液透析的患者的Bb水平显著高于各种肾脏疾病和胶原病患者。通过ELISA检测时，正常人血清中未检测到可检测量的末端补体复合物（TCC）。然而，正常人血清与血液透析膜在体外孵育后检测到了TCC。TCC存在于5例多囊肾患者首次血液透析期间采集的相同血浆样本中。这表明血液透析膜激活补体主要通过替代途径介导，并且这种激活会形成TCC和过敏毒素等产物。
