Kojima H, Ohi H, Seki M, Hatano M
Nihon Jinzo Gakkai Shi. 1989 Sep;31(9):925-31.
In this study we examined the role of the complement system in acute glomerulonephritis (AGN). Breakdown products of complements (iC3b, C4d and Bb) and Terminal complement complex (TCC, SC5b-9 complex) in plasma samples were measured by ELISA. The microassay plates were coated with monoclonal antibodies which bind specifically to human iC3b, C4d, Bb and SC5b-9 complex. This assay accurately quantitates small amounts of in vivo complement activation. The plasma samples were drawn from patients with AGN and other glomerulonephritis. There were some patients with various glomerulonephritis whose plasma iC3b, C4d and Bb concentrations were higher than those in normal human. However specificity was not found. The ratios iC3b/C3 and C4d/C4 were increased in the early stages of AGN, but plasma Bb concentrations revealed no significant changes. Plasma SC5b-9 complex concentrations were increased in the early stages of AGN. C3c, C3d, C4d and SC5b-9 were found to be localised in the glomeruli of those AGN patients. It is suggestive that in these cases of AGN (especially case 2) complement activation is predominantly mediated through the classical pathway and TCC is formed by this activation. This complement activation in the blood and the renal tissue is presumed to be involved in the initiation and progression of AGN.
在本研究中，我们检测了补体系统在急性肾小球肾炎（AGN）中的作用。采用酶联免疫吸附测定法（ELISA）检测血浆样本中补体的裂解产物（iC3b、C4d和Bb）以及末端补体复合物（TCC，即SC5b-9复合物）。微孔板用能特异性结合人iC3b、C4d、Bb和SC5b-9复合物的单克隆抗体包被。该检测方法能准确地定量体内少量的补体激活情况。血浆样本取自AGN患者和其他肾小球肾炎患者。有一些患有各种肾小球肾炎的患者，其血浆中iC3b、C4d和Bb的浓度高于正常人，但未发现特异性情况。在AGN早期，iC3b/C3和C4d/C4的比值升高，但血浆Bb浓度无显著变化。在AGN早期，血浆SC5b-9复合物浓度升高。在这些AGN患者的肾小球中发现了C3c、C3d、C4d和SC5b-9。这表明在这些AGN病例（尤其是病例2）中，补体激活主要通过经典途径介导，并且这种激活形成了TCC。血液和肾组织中的这种补体激活被认为与AGN的起始和进展有关。
