Li Xiang
Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou 450001, China.
Toxicol In Vitro. 2016 Oct;36:105-113. doi: 10.1016/j.tiv.2016.07.019. Epub 2016 Jul 26.
Cigarette smoke is a complex aerosol comprising particulate phase and gaseous vapour phase. The air-liquid interface exposure provides a possible technical means to implement whole smoke exposure for the assessment of tobacco products. In this review, the research progress in the in vitro toxicity testing of cigarette smoke based on the air-liquid interface exposure is summarized. The contents presented involve mainly cytotoxicity, genotoxicity, oxidative stress, inflammation, systems toxicology, 3D culture and cigarette smoke dosimetry related to cigarette smoke, as well as the assessment of electronic cigarette aerosol. Prospect of the application of the air-liquid interface exposure method in assessing the biological effects of tobacco smoke is discussed.
香烟烟雾是一种复杂的气溶胶，由颗粒相和气态蒸汽相组成。气液界面暴露为实施全烟雾暴露以评估烟草制品提供了一种可能的技术手段。在本综述中，总结了基于气液界面暴露的香烟烟雾体外毒性测试的研究进展。所呈现的内容主要涉及与香烟烟雾相关的细胞毒性、遗传毒性、氧化应激、炎症、系统毒理学、三维培养和香烟烟雾剂量学，以及电子烟气溶胶的评估。讨论了气液界面暴露方法在评估烟草烟雾生物学效应方面的应用前景。
