PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland.
Toxicol In Vitro. 2018 Oct;52:384-398. doi: 10.1016/j.tiv.2018.06.024. Epub 2018 Jul 9.
In vitro aerosol exposure of epithelial cells grown at the air-liquid interface is an experimental methodology widely used in respiratory toxicology. The exposure depends to a large part on the physicochemical properties of individual aerosol constituents, as they determine the transfer kinetics from the aerosol into the cells. We characterized the transfer of 70 cigarette smoke constituents from the smoke into aqueous samples exposed in the Vitrocell® 24/48 aerosol exposure system. The amounts of these compounds in the applied smoke were determined by trapping whole smoke in N,N-dimethylformamide and then compared with their amounts in smoke-exposed, phosphate-buffered saline, yielding compound specific delivery efficiencies. Delivery efficiencies of different smoke constituents differed by up to five orders of magnitude, which indicates that the composition of the applied smoke is not necessarily representative for the delivered smoke. Therefore, dose metrics for in vitro exposure experiments should, if possible, be based on delivered and not applied doses. A comparison to literature on in vivo smoke retention in the respiratory tract indicated that the same applies for smoke retention in the respiratory tract.
在气液界面培养的上皮细胞的体外气溶胶暴露是呼吸毒理学中广泛使用的一种实验方法。暴露在很大程度上取决于单个气溶胶成分的物理化学性质，因为它们决定了气溶胶向细胞内的转移动力学。我们描述了在 Vitrocell®24/48 气溶胶暴露系统中暴露的水溶液样品中 70 种香烟烟雾成分的转移情况。通过将整个烟雾捕获在 N,N-二甲基甲酰胺中，确定了这些化合物在应用烟雾中的含量，然后将其与暴露在烟雾中的磷酸盐缓冲盐水的含量进行比较，得出化合物特异性的传递效率。不同烟雾成分的传递效率差异可达五个数量级，这表明所应用的烟雾的组成不一定代表所传递的烟雾。因此，如果可能的话，体外暴露实验的剂量指标应该基于传递的而不是应用的剂量。与呼吸道内体内烟雾保留的文献进行比较表明，这同样适用于呼吸道内的烟雾保留。
