Junager Nina P L, Kongsted Jacob, Astakhova Kira
Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
Sensors (Basel). 2016 Jul 27;16(8):1173. doi: 10.3390/s16081173.
Nucleic acid mutations are of tremendous importance in modern clinical work, biotechnology and in fundamental studies of nucleic acids. Therefore, rapid, cost-effective and reliable detection of mutations is an object of extensive research. Today, Förster resonance energy transfer (FRET) probes are among the most often used tools for the detection of nucleic acids and in particular, for the detection of mutations. However, multiple parameters must be taken into account in order to create efficient FRET probes that are sensitive to nucleic acid mutations. In this review; we focus on the design principles for such probes and available computational methods that allow for their rational design. Applications of advanced, rationally designed FRET probes range from new insights into cellular heterogeneity to gaining new knowledge of nucleic acid structures directly in living cells.
核酸突变在现代临床工作、生物技术以及核酸基础研究中具有极其重要的意义。因此，快速、经济高效且可靠地检测突变是广泛研究的目标。如今，荧光共振能量转移（FRET）探针是检测核酸尤其是检测突变最常用的工具之一。然而，为了创建对核酸突变敏感的高效FRET探针，必须考虑多个参数。在本综述中，我们重点关注此类探针的设计原则以及可用于其合理设计的计算方法。先进的、经过合理设计的FRET探针的应用范围从对细胞异质性的新见解到直接在活细胞中获取核酸结构的新知识。
