Stanbridge E J, Wilkinson J
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1466-9. doi: 10.1073/pnas.75.3.1466.
Human cell hybrids derived from malignant HeLa and normal fibroblast parental cells expressed many of the transformed properties of the HeLa parent but their tumor-producing capability was suppressed. Hybrids derived from HeLa/HeLa fusions retained both their transformed and malignant phenotypes. Thus, an apparent separation of the control of the transformed versus malignant phenotype is indicated. Furthermore, several transformed properties--including lack of density-dependent inhibition of growth, lectin agglutination, lowered requirement for serum growth factors, and anchorage independence--are expressed coordinately in the nontumorigenic hybrids. This finding suggests that none of these properties by themselves, or in concert, endows a cell with tumorigenic potential.
源自恶性海拉细胞和正常成纤维细胞亲本细胞的人类细胞杂种表现出许多海拉亲本细胞的转化特性，但其产生肿瘤的能力受到抑制。源自海拉细胞/海拉细胞融合的杂种保留了它们的转化表型和恶性表型。因此，表明转化表型与恶性表型的控制存在明显分离。此外，几种转化特性——包括缺乏生长的密度依赖性抑制、凝集素凝集、对血清生长因子的需求降低以及锚定非依赖性——在非致瘤性杂种中协同表达。这一发现表明，这些特性单独或共同都不会赋予细胞致瘤潜力。
