一种新型可视化等温核酸扩增检测法与聚合酶链反应及皮肤切片分析用于人类盘尾丝虫病诊断的比较
Comparison of a new visual isothermal nucleic acid amplification test with PCR and skin snip analysis for diagnosis of onchocerciasis in humans.
作者信息
Alhassan Andy, Osei-Atweneboana Mike Y, Kyeremeh Kwadwo F, Poole Catherine B, Li Zhiru, Tettevi Edward, Tanner Nathan A, Carlow Clotilde K S
机构信息
New England Biolabs, Ipswich, MA, USA.
Council for Scientific and Industrial Research, Water Research Institute, Accra, Ghana.
出版信息
Mol Biochem Parasitol. 2016 Nov-Dec;210(1-2):10-12. doi: 10.1016/j.molbiopara.2016.07.006. Epub 2016 Jul 26.
Accurate, simple and affordable diagnostics are needed to detect Onchocerca volvulus infection in humans. A newly developed colorimetric loop-mediated isothermal amplification (LAMP) assay was compared to PCR and skin snip analysis for diagnosis of onchocerciasis. The robustness and simplicity of the assay indicates that it may be a useful field tool for surveillance in endemic countries.
需要准确、简单且经济实惠的诊断方法来检测人类的盘尾丝虫感染。将一种新开发的比色环介导等温扩增（LAMP）检测法与聚合酶链反应（PCR）和皮肤活检分析进行比较，以诊断盘尾丝虫病。该检测法的稳健性和简易性表明，它可能是流行国家进行监测的一种有用的现场工具。
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