Garg Nupur, Ahmad Farhan Jalees, Kar Sudeshna
Department of Pharmaceutics, School of Pharmaceutical Sciences & Research, Jamia Hamdard, New Delhi, India.
Department of Molecular Medicine, School of Interdisciplinary Science & Technology, Jamia Hamdard, New Delhi, India.
Curr Res Microb Sci. 2022 Feb 23;3:100120. doi: 10.1016/j.crmicr.2022.100120. eCollection 2022.
Loop-mediated isothermal amplification (LAMP) method has been demonstrated to bea reliable and robust method for detection and identification of viral and microbial pathogens. LAMP method of amplification, coupled with techniques for easy detection of amplicons, makes a simple-to-operate and easy-to-read molecular diagnostic tool for both laboratory and on-field settings. Several LAMP-based diagnostic kits and assays have been developed that are specifically targeted against a variety of pathogens. With the growing needs of the demanding molecular diagnostic industry, many technical advances have been made over the years by combining the basic LAMP principle with several other molecular approaches like real-time detection, multiplex methods, chip-based assays.This has resulted in enhancing thethe sensitivity and accuracy of LAMP for more rigorous and wide-ranging pathogen detection applications. This review summarizes the current developments in LAMP technique and their applicability in present and future disease diagnosis.
环介导等温扩增（LAMP）方法已被证明是一种用于检测和鉴定病毒及微生物病原体的可靠且强大的方法。LAMP扩增方法，再加上易于检测扩增产物的技术，使其成为一种在实验室和现场环境中都易于操作且易于读取结果的分子诊断工具。已经开发出了几种基于LAMP的诊断试剂盒和检测方法，它们专门针对多种病原体。随着对要求苛刻的分子诊断行业需求的不断增长，多年来通过将基本的LAMP原理与实时检测、多重方法、基于芯片的检测等其他几种分子方法相结合，取得了许多技术进步。这提高了LAMP在更严格和广泛的病原体检测应用中的灵敏度和准确性。本综述总结了LAMP技术的当前发展及其在当前和未来疾病诊断中的适用性。
