Yalley Akua K, Ahiatrogah Selasie, Kafintu-Kwashie Anna A, Amegatcher Gloria, Prah Diana, Botwe Akua K, Adusei-Poku Mildred A, Obodai Evangeline, Nii-Trebi Nicholas I
Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Accra P.O. Box KB 143, Ghana.
Department of Obstetrics and Gynaecology, College of Medicine, Pan African University of Life and Earth Sciences Institute, University of Ibadan, Ibadan P.O. Box 22133, Nigeria.
Curr Issues Mol Biol. 2022 Sep 21;44(10):4367-4385. doi: 10.3390/cimb44100300.
Infectious diseases significantly impact the health status of developing countries. Historically, infectious diseases of the tropics especially have received insufficient attention in worldwide public health initiatives, resulting in poor preventive and treatment options. Many molecular tests for human infections have been established since the 1980s, when polymerase chain reaction (PCR) testing was introduced. In spite of the substantial innovative advancements in PCR technology, which currently has found wide application in most viral pathogens of global concern, the development and application of molecular diagnostics, particularly in resource-limited settings, poses potential constraints. This review accessed data from sources including , , the , as well as reports from the World Health Organization's Annual Meeting on infectious diseases and examined these for current molecular approaches used to identify, monitor, or investigate some neglected tropical infectious diseases. This review noted some growth efforts in the development of molecular techniques for diagnosis of pathogens that appear to be common in resource limited settings and identified gaps in the availability and applicability of most of these molecular diagnostics, which need to be addressed if the One Health goal is to be achieved.
传染病对发展中国家的健康状况有着重大影响。从历史上看，热带地区的传染病在全球公共卫生倡议中尤其受到的关注不足，导致预防和治疗手段匮乏。自20世纪80年代引入聚合酶链反应（PCR）检测以来，已经建立了许多针对人类感染的分子检测方法。尽管PCR技术取得了重大的创新性进展，目前已在大多数全球关注的病毒病原体中得到广泛应用，但分子诊断技术的开发和应用，尤其是在资源有限的环境中，仍存在潜在限制。本综述从包括……、……、……以及世界卫生组织传染病年会报告等来源获取数据，并对这些数据进行研究，以了解用于识别、监测或调查一些被忽视的热带传染病的当前分子方法。本综述指出，在开发用于诊断资源有限环境中常见病原体的分子技术方面已做出了一些努力，并确定了大多数这些分子诊断方法在可用性和适用性方面存在的差距，如果要实现“同一个健康”目标，这些差距需要得到解决。
