Schoevers Eric J, Santos Regiane R, Fink-Gremmels Johanna, Roelen Bernard A J
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, The Netherlands.
Institute for Risk Assessment Sciences, Division Veterinary Pharmacology, Pharmacotherapy and Toxicology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, The Netherlands.
Reprod Toxicol. 2016 Oct;65:159-169. doi: 10.1016/j.reprotox.2016.07.017. Epub 2016 Jul 27.
Beauvericin (BEA) is one of many toxins produced by Fusarium species that contaminate feed materials. The aim of this study was to assess its effects on porcine oocyte maturation and preimplantation embryo development. Cumulus-oocyte-complexes and developing embryos were exposed to BEA and cultured until the blastocyst stage. Cumulus cells, oocytes and embryos were examined for viability, progesterone synthesis, multidrug resistance protein (MDR1), ATP content and gene expression related to MDR1 function, oxidative phosphorylation, steroidogenesis and apoptosis. BEA was toxic in embryos, oocytes and cumulus cells at concentrations exceeding 0.5μM, and embryos were most vulnerable after the four-cell stage. Since BEA exerted different effects in embryos, oocytes and cumulus cells, the toxic mechanism is suggested to involve different pathways. Currently there are no consistent data on adverse effects of BEA in pig farms.
白僵菌素（BEA）是污染饲料原料的镰刀菌属产生的多种毒素之一。本研究的目的是评估其对猪卵母细胞成熟和植入前胚胎发育的影响。将卵丘-卵母细胞复合体和发育中的胚胎暴露于白僵菌素中，并培养至囊胚阶段。检测卵丘细胞、卵母细胞和胚胎的活力、孕酮合成、多药耐药蛋白（MDR1）、ATP含量以及与MDR1功能、氧化磷酸化、类固醇生成和凋亡相关的基因表达。当浓度超过0.5μM时，白僵菌素对胚胎、卵母细胞和卵丘细胞具有毒性，并且胚胎在四细胞阶段后最为脆弱。由于白僵菌素在胚胎、卵母细胞和卵丘细胞中产生不同的影响，提示其毒性机制涉及不同的途径。目前关于养猪场中白僵菌素不良影响的相关数据并不一致。
