Lee Jee-Wook, Yun Hui-Suk, Nakano Takayoshi
1 School of Advanced Materials Engineering, Kookmin University , Seoul, Korea.
2 Powder and Ceramics Division, Korea Institute of Materials Science , Changwon, Korea.
Tissue Eng Part C Methods. 2016 Sep;22(9):856-63. doi: 10.1089/ten.TEC.2016.0133. Epub 2016 Aug 22.
Changes in the biological apatite (BAp) c-axis orientation were investigated as a bone quality parameter in bone regeneration using hydroxyapatite/poly ɛ-caprolactone (HA/PCL) composite scaffolds. Three-dimensional (3D) HA/PCL composite scaffolds were fabricated using a layer manufacturing process in three grid sizes (200-, 600-, and 1000 μm) and grafted into the forearm ulna of New Zealand white rabbits. The cross-sectional areas of the bones regenerated from the scaffolds with 600- and 1000-μm grid sizes were significantly larger than those from the scaffold with 200-μm grid sizes, whereas bone mineral density in the regenerated regions did not differ between the three grid sizes. Moreover, the BAp c-axis orientation in the bones regenerated from the scaffolds with grid sizes of 600- and 1000 μm was not significantly different; however, both scaffolds showed enhanced BAp orientation, although the degree of BAp orientation was lower than that in intact bones. In conclusion, HA/PCL composite 3D scaffolds with 600- and 1000-μm grid sizes induced BAp c-axis orientation and showed good bone regeneration behavior in vivo.
作为骨再生中的一个骨质量参数，研究了生物磷灰石（BAp）c轴取向的变化，该研究使用羟基磷灰石/聚ε-己内酯（HA/PCL）复合支架。采用分层制造工艺制备了三种网格尺寸（200、600和1000μm）的三维（3D）HA/PCL复合支架，并将其植入新西兰白兔的前臂尺骨。网格尺寸为600和1000μm的支架再生骨的横截面积显著大于网格尺寸为200μm的支架，而三种网格尺寸的再生区域的骨矿物质密度没有差异。此外，网格尺寸为600和1000μm的支架再生骨中的BAp c轴取向没有显著差异；然而，尽管BAp取向程度低于完整骨，但两种支架均显示出增强的BAp取向。总之，网格尺寸为600和1000μm的HA/PCL复合3D支架诱导了BAp c轴取向，并在体内表现出良好的骨再生行为。
