Department of Orthopedics, Shanghai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
Int J Nanomedicine. 2012;7:3123-31. doi: 10.2147/IJN.S31162. Epub 2012 Jul 10.
A bioactive composite of nano calcium-deficient apatite (n-CDAP) with an atom molar ratio of calcium to phosphate (Ca/P) of 1.50 and poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) (PCL-PEG-PCL) was synthesized, and a composite scaffold was fabricated. The composite scaffolds with 40 wt% n-CDAP contained well interconnected macropores around 400 μm, and exhibited a porosity of 75%. The weight-loss ratio of the n-CDAP/PCL-PEG-PCL was significantly greater than nano hydroxyapatite (n-HA, Ca/P = 1.67)/PCL-PEG-PCL composite scaffolds during soaking into phosphate-buffered saline (pH 7.4) for 70 days, indicating that n-CDAP-based composite had good degradability compared with n-HA. The viability ratio of MG-63 cells was significantly higher on n-CDAP than n-HA-based composite scaffolds at 3 and 5 days. In addition, the alkaline phosphatase activity of the MG-63 cells cultured on n-CDAP was higher than n-HA-based composite scaffolds at 7 days. Histological evaluation showed that the introduction of n-CDAP into PCL-PEG-PCL enhanced the efficiency of new bone formation when the composite scaffolds were implanted into rabbit bone defects. The results suggested that the n-CDAP-based composite exhibits good biocompatibility, biodegradation, and osteogenesis in vivo.
合成了原子摩尔比为钙磷（Ca/P）1.50 的纳米缺钙磷灰石（n-CDAP）与聚（ε-己内酯）-聚乙二醇-聚（ε-己内酯）（PCL-PEG-PCL）的生物活性复合材料，并制备了复合材料支架。n-CDAP 含量为 40wt%的复合支架具有约 400μm 的相互连通的大孔，孔隙率为 75%。在 pH 值为 7.4 的磷酸盐缓冲盐溶液中浸泡 70 天后，n-CDAP/PCL-PEG-PCL 的失重率明显大于纳米羟基磷灰石（n-HA，Ca/P=1.67）/PCL-PEG-PCL 复合支架，表明与 n-HA 相比，n-CDAP 基复合材料具有更好的降解性。在第 3 天和第 5 天，MG-63 细胞在 n-CDAP 上的存活率明显高于 n-HA 基复合材料支架。此外，在第 7 天，在 n-CDAP 上培养的 MG-63 细胞的碱性磷酸酶活性高于 n-HA 基复合材料支架。组织学评价表明，当将 n-CDAP 引入 PCL-PEG-PCL 中时，复合支架植入兔骨缺损中可提高新骨形成的效率。结果表明，n-CDAP 基复合材料具有良好的生物相容性、体内可降解性和成骨活性。
