基于还原态碳点过氧化物酶样活性的葡萄糖检测。
Detection of glucose based on the peroxidase-like activity of reduced state carbon dots.
机构信息
Key Laboratory on Luminescent and Real-Time Analytic Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Key Laboratory on Luminescent and Real-Time Analytic Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
出版信息
Talanta. 2016 Oct 1;159:122-126. doi: 10.1016/j.talanta.2016.06.012. Epub 2016 Jun 7.
It was found that reduced state carbon dots (r-CDs) possessed intrinsic peroxidase-like activity, and could catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to produce a color reaction. The effects of temperature, pH, incubating time and the concentration of H2O2 and TMB on catalytic activity of r-CDs were investigated. Finally we calculated the kinetic constant was ca. 0.00729min(-1) and applied r-CDs to glucose sensing by coupling glucose oxidase. As low as 2µM H2O2 could be detected with a linear range from 0.010 to 0.40mM via this method. This study offered a simple, sensitive, and high selectivity method for glucose determination even in serum.
研究发现，还原态碳点（r-CDs）具有固有过氧化物酶样活性，能够在 H2O2 的作用下将 3,3',5,5'-四甲基联苯胺（TMB）催化氧化产生颜色反应。考察了温度、pH 值、孵育时间以及 H2O2 和 TMB 浓度对 r-CDs 催化活性的影响。最后，我们计算出动力学常数约为 0.00729min(-1)，并将 r-CDs 应用于葡萄糖氧化酶偶联的葡萄糖传感。通过该方法，可检测到低至 2µM 的 H2O2，线性范围为 0.010 至 0.40mM。该研究为葡萄糖的测定提供了一种简单、灵敏、高选择性的方法，即使在血清中也是如此。
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