Bioprocess and Bioenergy Laboratory, Department of Microbiology, Central University of Rajasthan, Kishangarh 305817, India.
Department of Engineering, University of Campania "Luigi Vanvitelli", Real Casa dell'Annunziata, Via Roma 29, 81031 Aversa, Italy.
Int J Environ Res Public Health. 2022 Mar 19;19(6):3674. doi: 10.3390/ijerph19063674.
The major downfalls of the microalgal biorefinery are low volume of high value product accumulation, low biomass productivity and high cultivation costs. Here, we aimed to improve the biomass productivity of the industrially relevant sp. BDUG 100241 strain. The growth of sp. BDUG 100241 was investigated under different cultivations conditions, including photoautotrophic (with light), mixotrophic (1% glucose, with light) and heterotrophic (1% glucose, without light). Among them, sp. BDUG100241 showed the highest growth in the mixotrophic condition. Under different (1%) carbon sources' supplementation, including glucose, sodium acetate, glycerol, citric acid and methanol, sp. BDUG100241 growth was tested. Among them, sodium acetate was found to be most suitable carbon source for sp. BDUG 100241 growth, biomass (1.67 ± 0.18 g/L) and biomolecule productivity. From the different concentrations of sodium acetate (0, 2.5, 5.0, 7.5 and 10 g/L) tested, the maximum biomass production of 2.40 ± 0.20 g/L with the biomass productivity of 95 ± 5.00 mg/L/d was measured from 7.5 g/L in sodium acetate. The highest total lipid (53.50 ± 1.70%) and total carotenoids (0.75 ± 0.01 µg/mL) contents were observed at the concentration of 7.5 g/L and 5.0 g/L of sodium acetate as a carbon source, respectively. In conclusion, the mixotrophic growth condition containing 7.5 g/L of sodium acetate showed the maximum biomass yield and biomolecule accumulation compared to other organic carbon sources.
微藻生物炼制的主要缺点是高价值产品积累量低、生物量生产率低和培养成本高。在这里，我们旨在提高工业相关 sp 的生物量生产率。BDUG 100241 菌株。在不同的培养条件下研究了 sp 的生长。BDUG 100241，包括自养（有光）、混合营养（1%葡萄糖，有光）和异养（1%葡萄糖，无光）。其中，sp。BDUG100241 在混合营养条件下生长最快。在不同（1%）碳源补充下，包括葡萄糖、醋酸钠、甘油、柠檬酸和甲醇，sp。BDUG100241 的生长情况进行了测试。其中，发现醋酸钠是最适合 sp 的碳源。BDUG 100241 生长，生物量（1.67±0.18 g/L）和生物分子生产率。从不同浓度的醋酸钠（0、2.5、5.0、7.5 和 10 g/L）测试中，最大生物量为 2.40±0.20 g/L，生物量生产率为 95±5.00 mg/L/d，从 7.5 g/L 的醋酸钠中得到。在 7.5 g/L 和 5.0 g/L 的醋酸钠作为碳源时，分别观察到最高的总脂质（53.50±1.70%）和总类胡萝卜素（0.75±0.01 µg/mL）含量。总之，与其他有机碳源相比，含有 7.5 g/L 醋酸钠的混合营养生长条件显示出最大的生物量产量和生物分子积累。
