采用分子印迹固相萃取-高效液相色谱荧光检测法测定胶州湾中的环丙沙星。
Determination of ciprofloxacin in Jiaozhou Bay using molecularly imprinted solid-phase extraction followed by high-performance liquid chromatography with fluorescence detection.
机构信息
Marine College, Shandong University, Weihai 264209, China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100,China.
出版信息
Mar Pollut Bull. 2016 Oct 15;111(1-2):411-417. doi: 10.1016/j.marpolbul.2016.07.034. Epub 2016 Jul 27.
A high selective pre-treatment method for the cleanup and preconcentration of ciprofloxacin in natural seawater samples was developed based on molecularly imprinted solid-phase extraction (MISPE). The ciprofloxacin imprinted polymers were synthesized and the characteristics of obtained polymers were evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy and binding experiments. The imprinted materials showed high adsorption ability for ciprofloxacin and were applied as special solid-phase extraction sorbents for selective separation of ciprofloxacin. An off-line MISPE procedure was optimized and the developed MISPE method allowed direct purification and enrichment of the ciprofloxacin from the aqueous samples prior to high-performance liquid chromatography analysis. The recoveries of spiked seawater on the MISPE cartridges ranged from 75.2 to 112.4% and the relative standard deviations were less than 4.46%. Five seawater samples from Jiaozhou Bay were analyzed and ciprofloxacin was detected in two samples with the concentrations of 0.24 and 0.38μgL(-1), respectively.
基于分子印迹固相萃取（MISPE），开发了一种用于从天然海水中样品中净化和预浓缩环丙沙星的高选择性预处理方法。合成了环丙沙星印迹聚合物，并通过扫描电子显微镜、傅里叶变换红外光谱和结合实验对获得的聚合物的特性进行了评估。印迹材料对环丙沙星具有高吸附能力，并用作特殊固相萃取吸附剂，用于环丙沙星的选择性分离。优化了离线 MISPE 程序，所开发的 MISPE 方法允许在高效液相色谱分析之前直接从水样中净化和富集环丙沙星。在 MISPE 小柱上对加标海水的回收率在 75.2%至 112.4%之间，相对标准偏差小于 4.46%。分析了来自胶州湾的 5 个海水样品，在两个样品中检测到环丙沙星，浓度分别为 0.24 和 0.38μg/L。
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