采用分子印迹固相萃取-高效液相色谱-二极管阵列检测法测定胶州湾磺胺嘧啶
Determination of sulfadiazine in Jiaozhou Bay using molecularly imprinted solid-phase extraction followed by high-performance liquid chromatography with a diode-array detector.
机构信息
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China; Marine College, Shandong University at Weihai, Weihai 264209, PR China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, PR China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Apr 15;957:53-9. doi: 10.1016/j.jchromb.2014.02.053. Epub 2014 Mar 12.
A highly selective sample cleanup procedure featuring molecularly imprinted solid-phase extraction (MISPE) was developed for the isolation and determination of sulfadiazine (SDZ) in seawater samples from Jiaozhou Bay, China. The molecularly imprinted polymer (MIP) was prepared using SDZ as the template molecule and methacrylic acid as the functional monomer. The MIP was used as a selective sorbent for the solid-phase extraction of SDZ. An off-line MISPE method followed by high-performance liquid chromatography (HPLC) with diode-array detection was established for the analysis of SDZ. Good linearity of the MISPE column for SDZ standard solutions was obtained within 0-200μgL(-1) (R(2)>0.99). The recoveries of spiked seawater samples were satisfactory as high as 88%. Finally, seven samples in Jiaozhou Bay were determined and there was no sulfadiazine found except #2 and #5 sample. The concentrations were respectively 0.33μgL(-1) and 0.28μgL(-1), and the relative standard deviations were 1.35% and 4.13% (n=3).
建立了一种高选择性的样品净化方法，即分子印迹固相萃取（MISPE），用于分离和测定中国胶州湾海水中的磺胺嘧啶（SDZ）。该分子印迹聚合物（MIP）以磺胺嘧啶为模板分子，甲基丙烯酸为功能单体进行制备。MIP 被用作固相萃取磺胺嘧啶的选择性吸附剂。采用离线 MISPE 方法，结合二极管阵列检测高效液相色谱法（HPLC），建立了磺胺嘧啶的分析方法。磺胺嘧啶标准溶液在 0-200μgL(-1)（R(2)>0.99）范围内具有良好的 MISPE 柱线性。加标海水样品的回收率高达 88%。最后，对胶州湾的 7 个水样进行了测定，除 #2 和 #5 样品外，其他样品均未检出磺胺嘧啶。浓度分别为 0.33μgL(-1)和 0.28μgL(-1)，相对标准偏差分别为 1.35%和 4.13%（n=3）。
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