Lauersen Kyle J, Baier Thomas, Wichmann Julian, Wördenweber Robin, Mussgnug Jan H, Hübner Wolfgang, Huser Thomas, Kruse Olaf
Bielefeld University, Faculty of Biology, Center for Biotechnology (CeBiTec), Universitätsstrasse 27, 33615 Bielefeld, Germany.
Biomolecular Photonics, Department of Physics, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany.
Metab Eng. 2016 Nov;38:331-343. doi: 10.1016/j.ymben.2016.07.013. Epub 2016 Jul 27.
The heterologous expression of terpene synthases in microbial hosts has opened numerous possibilities for bioproduction of desirable metabolites. Photosynthetic microbial hosts present a sustainable alternative to traditional fermentative systems, using freely available (sun)light and carbon dioxide as inputs for bio-production. Here, we report the expression of a patchoulol synthase from Pogostemon cablin Benth in the model green microalga Chlamydomonas reinhardtii. The sesquiterpenoid patchoulol was produced from the alga and was used as a marker of sesquiterpenoid production capacity. A novel strategy for gene loading was employed and patchoulol was produced up to 922±242µgg CDW in six days. We additionally investigated the effect of carbon source on sesquiterpenoid productivity from C. reinhardtii in scale-up batch cultivations. It was determined that up to 1.03mgL sesquiterpenoid products could be produced in completely photoautotrophic conditions and that the alga exhibited altered sesquiterpenoid production metabolism related to carbon source.
萜类合酶在微生物宿主中的异源表达为生物生产所需代谢产物开辟了众多可能性。光合微生物宿主为传统发酵系统提供了一种可持续的替代方案，利用免费可得的（太阳）光和二氧化碳作为生物生产的输入物。在此，我们报道了来自广藿香的广藿香醇合酶在模式绿色微藻莱茵衣藻中的表达。该藻类产生了倍半萜广藿香醇，并将其用作倍半萜生产能力的标志物。采用了一种新的基因导入策略，六天内广藿香醇产量高达922±242µg g CDW。我们还在放大分批培养中研究了碳源对莱茵衣藻倍半萜生产力的影响。结果表明，在完全光自养条件下，可产生高达1.03mg L的倍半萜产物，并且该藻类表现出与碳源相关的倍半萜生产代谢变化。
