光交联细胞膜模拟壳聚糖水凝胶的制备、表征及蛋白质吸附。
Preparation, characterization and protein sorption of photo-crosslinked cell membrane-mimicking chitosan-based hydrogels.
机构信息
Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China.
Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, PR China.
出版信息
Carbohydr Polym. 2016 Oct 20;151:237-244. doi: 10.1016/j.carbpol.2016.05.067. Epub 2016 May 20.
Photocrosslinkable biomimetic chitosan derivative, glycidyl methacrylate-phosphorylcholine-chitosan (PCCs-GMA) was synthesized through the combination of Atherton-Todd reaction for coupling phosphorylcholine and ring opening reaction of epoxides for attaching GMA, and confirmed by (1)H and (31)P NMR and Fourier transform infrared (FTIR) spectroscopy. The photo-crosslinking reaction of PCCs-GMA with different degree of substitution (DS) of GMA allowed the formation of biomimetic hydrogels with tunable mechanical and swelling properties. Cold crystallization behaviors ascribed to their restrained freezing bound water were investigated using differential scanning calorimetry (DSC). The rheological and swelling behaviors, hemolysis as well as protein sorption of PCCs-GMA hydrogels were investigated in terms of the DS of GMA, using fibrinogen, bovine serum albumin and lysozyme as model proteins. Low irreversible protein sorption and non hemolytic results indicated that photo-crosslinked PCCs-GMA hydrogels may offer a promising candidate material with resistance to protein fouling in biomedical applications.
通过 Atherton-Todd 反应偶联磷酰胆碱和环氧化物开环反应接枝 GMA 合成了光交联仿生壳聚糖衍生物甲基丙烯酰氧乙基磷酸胆碱-壳聚糖（PCCs-GMA），并通过（1）H 和（31）P NMR 和傅里叶变换红外（FTIR）光谱进行了确认。不同取代度（DS）的 GMA 的 PCCs-GMA 的光交联反应允许形成具有可调机械和溶胀性能的仿生水凝胶。使用差示扫描量热法（DSC）研究了归因于其受限的结合水的冷结晶行为。使用纤维蛋白原、牛血清白蛋白和溶菌酶作为模型蛋白，根据 GMA 的 DS 研究了 PCCs-GMA 水凝胶的流变学和溶胀行为、溶血和蛋白质吸附。低不可逆蛋白质吸附和非溶血结果表明，光交联 PCCs-GMA 水凝胶在生物医学应用中可能是一种具有抗蛋白质污染能力的有前途的候选材料。
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