Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, India.
Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, India.
Carbohydr Polym. 2016 Oct 20;151:335-347. doi: 10.1016/j.carbpol.2016.05.088. Epub 2016 May 25.
Novel silk fibroin (SF) and carboxymethyl cellulose (CMC) composite nanofibrous scaffold (SFC) were developed to investigate their ability to nucleate bioactive nanosized calcium phosphate (Ca/P) by biomineralization for bone tissue engineering application. The composite nanofibrous scaffold was prepared by free liquid surface electrospinning method. The developed composite nanofibrous scaffold was observed to control the size of Ca/P particle (≤100nm) as well as uniform nucleation of Ca/P over the surface. The obtained nanofibrous scaffolds were fully characterized for their functional, structural and mechanical property. The XRD and EDX analysis depicted the development of apatite like crystals over SFC scaffolds of nanospherical in morphology and distributed uniformly throughout the surface of scaffold. Additionally, hydrophilicity as a measure of contact angle and water uptake capacity is higher than pure SF scaffold representing the superior cell supporting property of the SF/CMC scaffold. The effect of biomimetic Ca/P on osteogenic differentiation of umbilical cord blood derived human mesenchymal stem cells (hMSCs) studied in early and late stage of differentiation shows the improved osteoblastic differentiation capability as compared to pure silk fibroin. The obtained result confirms the positive correlation of alkaline phosphatase activity, alizarin staining and expression of runt-related transcription factor 2, osteocalcin and type1 collagen representing the biomimetic property of the scaffolds. Thus, the developed composite has been demonstrated to be a potential scaffold for bone tissue engineering application.
新型丝素蛋白（SF）和羧甲基纤维素（CMC）复合纳米纤维支架（SFC）的开发，旨在研究其通过生物矿化能力在骨组织工程应用中诱导生物活性纳米磷酸钙（Ca/P）的能力。复合纳米纤维支架通过自由液面膜电纺方法制备。观察到开发的复合纳米纤维支架能够控制 Ca/P 颗粒的尺寸（≤100nm）以及在表面上均匀地进行 Ca/P 的成核。对获得的纳米纤维支架进行了全面的功能、结构和机械性能表征。XRD 和 EDX 分析表明，在 SFC 支架上形成了具有纳米球形形态和均匀分布在支架表面的类磷灰石晶体。此外，作为接触角和吸水率的测量指标，亲水性高于纯 SF 支架，表明 SF/CMC 支架具有更好的细胞支持性能。在早期和晚期分化阶段研究仿生 Ca/P 对脐带血来源的人间充质干细胞（hMSCs）成骨分化的影响表明，与纯丝素蛋白相比，具有改善的成骨细胞分化能力。获得的结果证实碱性磷酸酶活性、茜素红染色和 runt 相关转录因子 2、骨钙素和 I 型胶原的表达呈正相关，表明支架具有仿生特性。因此，所开发的复合材料已被证明是骨组织工程应用的潜在支架。
