a Center of Excellence in Tissue Engineering, Department of Biotechnology and Medical Engineering , National Institute of Technology Rourkela , Rourkela , India.
J Biomater Sci Polym Ed. 2018 Nov;29(16):2011-2034. doi: 10.1080/09205063.2018.1523525. Epub 2018 Oct 29.
The development of bone tissue construct through tissue engineering approach offers a great promise in meeting the increasing demand for repair and regeneration of damaged and/or diseased bone tissue. For the generation of bone tissue engineered construct, polymer-ceramic composite matrices with nanostructure architecture and mesenchymal stem cells (hMSCs) of human origin are of prime requirement. Keeping these in view, in the present work a novel electrospun nanofibrous silk fibroin (SF)/carboxymethyl cellulose (CMC)/nano-bioglass (nBG) composite scaffold that mimics native bone extracellular matrix with appropriate composition was designed and fabricated by free liquid surface electrospinning technique. The scaffold possesses desired morphological, structural, biodegradability, bioactivity, surface roughness and mechanical properties thereby exhibited an excellent platform to support the growth of cells. The in-vitro culture of hMSCs over the developed scaffold has shown adhesion, proliferation and viability of cells, thus facilitated cell-scaffold construct generation and further extracellular bone matrix formation through osteogenic differentiation as evident from alkaline phosphatase activity, biomineralization, immunostaining and Runx2/osteocalcin expression assessment. Thus, the developed hMSCs seeded scaffold construct might be suitable for bone tissue engineering applications.
通过组织工程方法开发骨组织构建体在满足修复和再生受损和/或患病骨组织的日益需求方面具有巨大的潜力。为了生成骨组织工程构建体，需要具有纳米结构架构的聚合物-陶瓷复合材料基质和人源性间充质干细胞（hMSCs）。有鉴于此，本研究设计并通过自由液-面静电纺丝技术制备了一种新型的仿生天然骨细胞外基质的电纺纳米纤维丝素（SF）/羧甲基纤维素（CMC）/纳米生物玻璃（nBG）复合支架，具有适当的组成。支架具有所需的形态、结构、生物降解性、生物活性、表面粗糙度和机械性能，从而为细胞的生长提供了一个极好的平台。在开发的支架上进行 hMSCs 的体外培养表明细胞的黏附、增殖和活力，从而通过成骨分化促进细胞-支架构建体的生成和进一步的细胞外骨基质形成，这可以从碱性磷酸酶活性、生物矿化、免疫染色和 Runx2/骨钙素表达评估中得到证明。因此，开发的 hMSCs 接种支架构建体可能适用于骨组织工程应用。
