沙枣果肉多糖提取工艺优化、初步性质鉴定及体外免疫活性研究。
Extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp.
机构信息
College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China; College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China.
College of Sciences, Northwest A&F University, Yangling 712100, Shaanxi Province, China.
出版信息
Carbohydr Polym. 2016 Oct 20;151:348-357. doi: 10.1016/j.carbpol.2016.05.068. Epub 2016 May 20.
In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82±0.38%, which is in good agreement with the predicted value (9.93±0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70kDa and 4.39kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods.
本研究旨在探讨沙枣果肉多糖的提取优化、初步表征和体外免疫活性。采用 Box-Behnken 设计（BBD）的响应面法（RSM）优化提取工艺。最大 EAP 得率为 9.82±0.38%，与预测值（9.93±0.24%）吻合较好。通过 DEAE-52 纤维素和 Sephadex G-100 柱层析分离得到两种均一多糖 EAP-1a 和 EAP-1b，其分子量分别为 8.70kDa 和 4.39kDa。采用 HPLC、HPGPC 和 FT-IR 对其进行了表征。三种多糖（EAP、EAP-1a 和 EAP-1b）均可剂量依赖性地刺激巨噬细胞释放 NO，并增强 RAW 264.7 细胞的吞噬活性。此外，粗 EAP 组（400μg/mL）与阳性对照组（LPS）在巨噬细胞作用方面无显著差异。结果表明，EAP 具有开发为天然药物或保健品的潜力。
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