Chen Juncheng, Tian Shan, Shu Xiaoying, Du Hongtao, Li Na, Wang Junru
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Sciences, Northwest A & F University, Yangling 712100, Shaanxi, China.
College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi, China.
Int J Mol Sci. 2016 Jun 25;17(7):1011. doi: 10.3390/ijms17071011.
A response surface and Box-Behnken design approach was applied to augment polysaccharide extraction from the residue of Rhizoma gastrodiae. Statistical analysis revealed that the linear and quadratic terms for three variables during extraction exhibited obvious effects on extraction yield. The optimum conditions were determined to be a liquid-to-solid ratio of 54 mL/g, an extraction temperature of 74 °C, an extraction time of 66 min, and three extractions. These conditions resulted in a maximum Rhizoma gastrodiae polysaccharide (RGP) extraction yield of 6.11% ± 0.13%. Two homogeneous polysaccharides (RGP-1a and RGP-1b) were obtained using DEAE cellulose-52 and Sephadex G-100 columns. The preliminary characterization of RGP-1a and RGP-1b was performed using HPLC-RID, HPGPC, and FTIR. Tests of the immunological activity in vitro showed that the two polysaccharides could significantly stimulate macrophages to release NO and enhance phagocytosis in a dose-dependent manner. In particular, RGP-1b (200 μg/mL) and LPS (2 μg/mL) had almost the same influence on the NO production and phagocytic activity of RAW 264.7 macrophages (p > 0.05). All the data obtained indicate that RGP-1a and RGP-1b have the potential to be developed as a health food.
采用响应面法和Box-Behnken设计方法提高天麻残渣中多糖的提取率。统计分析表明，提取过程中三个变量的线性项和二次项对提取率有显著影响。确定最佳条件为料液比54 mL/g、提取温度74℃、提取时间66 min、提取三次。在此条件下，天麻多糖（RGP）的最大提取率为6.11%±0.13%。使用DEAE纤维素-52和Sephadex G-100柱获得了两种均一多糖（RGP-1a和RGP-1b）。采用HPLC-RID、HPGPC和FTIR对RGP-1a和RGP-1b进行了初步表征。体外免疫活性测试表明，这两种多糖能显著刺激巨噬细胞释放NO，并以剂量依赖的方式增强吞噬作用。特别是，RGP-1b（200μg/mL）和LPS（2μg/mL）对RAW 264.7巨噬细胞的NO产生和吞噬活性的影响几乎相同（p>0.05）。所有获得的数据表明，RGP-1a和RGP-1b有开发成保健食品的潜力。
