Giorgio A, Rambaldi M, Iaquinto G, Carrato F, Ambrosone L, Moles D A, Troise C
Istituto di Richerche Biologiche F. Redi Avellino, Italy.
Microbiologica. 1989 Apr;12(2):151-5.
A new method to evaluate lymphocyte response to mitogens is proposed. The method is based on the observation in fluorescence of the stimulated cells after their supravital staining on slides prestained with acridine orange in alcoholic solution. The results are expressed as Blast Index (IB). The method is very practical, easy to reproduce and shows a good correlation with 3H-thymidine uptake.
提出了一种评估淋巴细胞对有丝分裂原反应的新方法。该方法基于对用酒精溶液中的吖啶橙预染过的载玻片上的活体细胞进行超活染色后，对刺激细胞荧光的观察。结果以母细胞化指数（IB）表示。该方法非常实用，易于重复，并且与3H-胸腺嘧啶核苷摄取显示出良好的相关性。
