Gill C, Fischer C L, Wilkins B, Nakamura R
Med Instrum. 1976 Jan-Feb;10(1):9-15.
This rapid, automated, micromethod quantitates blastoid transformation using a supra vital stain, acridine orange, in quantitating the ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) before and after cell stimulation. Only 0.2 ml of culture containing 1.0 X 10(5) to 1.5 X 10(5) lymphocytes is required to obtain results every 5 minutes. These results include a total cell count and cell counts of the populations with increased RNA (I-RNA), and increased DNA (I-DNA). The I-RNA and I-DNA lymphocyte populations, as determined by the cytofluorometric method, correlate well with the accepted H3 uridine and C14 thymidine uptake methodology. The phytohemagglutinin dose response measurements by the 2 methods also correlate well. Blast cell counts, by Wright-Giemsa staining, showed near identity to the I-RNA cell population.
这种快速、自动化的微量方法使用一种超活染色剂吖啶橙来定量胚样转化，在细胞刺激前后对核糖核酸（RNA）和脱氧核糖核酸（DNA）进行定量。每5分钟只需0.2毫升含有1.0×10⁵至1.5×10⁵淋巴细胞的培养物就能获得结果。这些结果包括总细胞计数以及RNA增加（I-RNA）和DNA增加（I-DNA）的细胞群体计数。通过细胞荧光测定法确定的I-RNA和I-DNA淋巴细胞群体与公认的³H尿苷和¹⁴C胸苷摄取方法相关性良好。两种方法对植物血凝素剂量反应的测量也相关性良好。通过瑞氏-吉姆萨染色进行的原始细胞计数与I-RNA细胞群体几乎一致。
