Nakajima M, Kitazawa M, Oba K, Kitagawa Y, Toyoda Y
Biosafety Research Center, Foods, Drugs, and Pesticides (An-Pyo Center), Shizuoka-ken, Japan.
Mutat Res. 1989 Aug;223(4):399-402. doi: 10.1016/0165-1218(89)90095-5.
The effect of intraperitoneal injection (i.p.) versus oral gavage administration (p.o.) of potassium bromate was examined using the micronucleus test in 2 strains of male mice (MS/Ae and CD-1). First, a small acute toxicity test and a pilot micronucleus experiment were performed to determine the appropriate dose range and sampling time for the full-scale micronucleus test. The full-scale test was carried out using doses of 18.8, 37.5, 75, and 150 mg/kg in the i.p. test and of 37.5, 75, 150, and 300 mg/kg in the p.o. test. The sampling time was 24 h for both mouse strains. Potassium bromate induced micronucleated polychromatic erythrocytes (MNPCEs) dose-dependently by both routes of administration in both mouse strains. No distinct difference in route of administration was observed in the test with MS/Ae mice. In CD-1 mice more MNPCEs were induced by the i.p. route than by the p.o. route.
使用微核试验在2个品系的雄性小鼠（MS/Ae和CD-1）中检测了腹腔注射（i.p.）与灌胃给药（p.o.）溴酸钾的效果。首先，进行了一项小型急性毒性试验和一项预试验微核实验，以确定全面微核试验的合适剂量范围和采样时间。全面试验中，腹腔注射试验使用的剂量为18.8、37.5、75和150mg/kg，灌胃试验使用的剂量为37.5、75、150和300mg/kg。两个品系小鼠的采样时间均为24小时。在两个品系小鼠中，两种给药途径下溴酸钾均剂量依赖性地诱导了微核多染红细胞（MNPCEs）。在MS/Ae小鼠的试验中，未观察到给药途径的明显差异。在CD-1小鼠中，腹腔注射途径比灌胃途径诱导产生更多的MNPCEs。
