Sato S, Inui N, Ikeda Y, Hiraga Y
Toxicology Research Laboratories, Japan Tobacco Inc., Kanagawa.
Mutat Res. 1989 Aug;223(4):387-90. doi: 10.1016/0165-1218(89)90092-x.
Intraperitoneal (i.p.) injection and oral (p.o.) gavage were evaluated in the mouse micronucleus test with mitomycin C (MMC). The tests were carried out in 2 laboratories with the MS/Ae and CD-1 mouse strains. On the basis of a small-scale acute toxicity study and a pilot experiment, the full-scale micronucleus test was performed with a 24-h sampling time at doses of 1, 2, 4, and 8 mg/kg for both treatment routes. In both strains, a clear positive dose-response relation was shown by both routes. Although the frequency of micronucleated polychromatic erythrocytes (MNPCEs) was higher with i.p. on a mg/kg basis, this tendency was reversed when dose was expressed as a percentage of the LD50.
在使用丝裂霉素C（MMC）的小鼠微核试验中，对腹腔注射（i.p.）和口服灌胃（p.o.）进行了评估。试验在2个实验室使用MS/Ae和CD-1小鼠品系进行。基于小规模急性毒性研究和预试验，在两种给药途径下，以1、2、4和8mg/kg的剂量进行了24小时采样时间的全面微核试验。在两个品系中，两种途径均显示出明显的阳性剂量反应关系。虽然以mg/kg为基础时，腹腔注射的微核多染红细胞（MNPCEs）频率较高，但当剂量以LD50的百分比表示时，这种趋势发生了逆转。
