Bauchinger M, Kulka U, Schmid E
Institut für Strahlenbiologie, Gesellschaft für Strahlen-und Umweltforschung, Neuherberg/Munich, F.R.G.
Mutat Res. 1989 Jul;226(3):197-202. doi: 10.1016/0165-7992(89)90020-1.
3,4-Dichloroaniline (3,4-DCA), an intermediate in various chemical syntheses, has been detected as an environmental contaminant in surface waters and in the effluents from dye-manufacturing plants. Tested for clastogenicity in human lymphocytes in vitro the compound was inactive in the chromosome aberration assay yet exhibited a positive sister-chromatid exchange response in the presence of a mammalian metabolic activation system. Exposure of V79 Chinese hamster cells to 3,4-DCA caused a concentration-dependent increase in the incidence of spindle disturbances, predominantly of the initial c-mitotic type. The results indicate that 3,4-DCA might induce aneuploidy in mammalian cells by interaction with the mitotic apparatus.
3,4-二氯苯胺（3,4-DCA）是多种化学合成过程中的中间体，已在地表水和染料制造工厂的废水中被检测为环境污染物。该化合物在体外对人类淋巴细胞进行致断裂性测试时，在染色体畸变试验中无活性，但在存在哺乳动物代谢激活系统的情况下表现出阳性的姐妹染色单体交换反应。将V79中国仓鼠细胞暴露于3,4-DCA会导致纺锤体紊乱发生率呈浓度依赖性增加，主要为初始c-有丝分裂类型。结果表明，3,4-DCA可能通过与有丝分裂装置相互作用在哺乳动物细胞中诱导非整倍体。
