非人灵长类动物研究群体中肺炎克雷伯菌爆发期间分离株的遗传多样性。
Genetic diversity of Klebsiella pneumoniae isolates during an outbreak in a non-human primate research colony.
作者信息
Gozalo Alfonso S, Elkins William R, Lambert Lynn E, Stock Frida, Thomas Marvin L, Woodward Ruth A
机构信息
Comparative Medicine Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Laboratory of Malaria Immunology and Vaccinology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
出版信息
J Med Primatol. 2016 Dec;45(6):312-317. doi: 10.1111/jmp.12229. Epub 2016 Aug 1.
BACKGROUND
Klebsiella pneumoniae can be a serious pathogen in non-human primates, particularly Neotropical monkeys.
METHODS
During a K. pneumoniae outbreak in an owl monkey research colony, 13 K. pneumoniae isolates were DNA fingerprinted by automated repetitive extragenic palindromic-polymerase chain reaction and the profiles compared to isolates obtained from other non-human primate species during the same time period and isolates from previous outbreaks.
RESULTS
Eleven different types of K. pneumoniae were circulating in the owl monkey colony at the time of the outbreak. When comparing owl monkey isolates relatedness to previous colony outbreak isolates and squirrel monkey and capuchin monkey isolates, all were different.
CONCLUSIONS
These results agree with recent reports where K. pneumoniae nosocomial isolates in hospital settings can have high genetic diversity, and multiple strains can be circulating simultaneously. This potential genetic diversity should be considered when designing strategies for controlling K. pneumoniae outbreaks in captive non-human primate colonies.
背景
肺炎克雷伯菌可能是非人灵长类动物，尤其是新热带猴的一种严重病原体。
方法
在一个夜猴研究群体中发生肺炎克雷伯菌疫情期间，通过自动重复外源性回文聚合酶链反应对13株肺炎克雷伯菌分离株进行DNA指纹图谱分析，并将图谱与同期从其他非人灵长类物种获得的分离株以及先前疫情中的分离株进行比较。
结果
疫情爆发时，11种不同类型的肺炎克雷伯菌在夜猴群体中传播。将夜猴分离株与先前群体疫情分离株以及松鼠猴和卷尾猴分离株的亲缘关系进行比较时，发现它们均不相同。
结论
这些结果与近期的报告一致，即在医院环境中，肺炎克雷伯菌医院感染分离株具有高度的遗传多样性，并且多种菌株可能同时传播。在设计控制圈养非人灵长类群体中肺炎克雷伯菌疫情的策略时，应考虑这种潜在的遗传多样性。
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